Polysaccharides in Sipunculus nudus: Extraction condition optimization and antioxidant activities

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• 作者：Qin Zhang ; Lanfang Dong ; Tong Tong ; Qingchao Wang…
• 关键词：Key wordsSipunculus nudus polysaccharide ; extraction condition ; antioxidant activities
• 刊名：Journal of Ocean University of China
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：16
• 期：1
• 页码：74-80
• 全文大小：
• 刊物主题：Oceanography; Meteorology;
• 出版者：Science Press
• ISSN：1993-5021
• 卷排序：16

文摘

Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides (SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60°C, solid-liquid ratio 1:6 g mL−1, duration 5 h, and alkali (NaOH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.