Analysis of Fish DNA Biomarkers as a Molecular-Level Approach for Ecological Health Assessments in an Urban Stream
详细信息 查看全文
- 作者：Nami Kang (1)
Han-il Kang (1)
Kwang-Guk An (1) - 关键词：Pseudogobio esocinus ; Zacco platypus ; Danio rerio ; Genotoxicity ; Stream environmental health assessment
- 刊名：Bulletin of Environmental Contamination and Toxicology
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：93
- 期：5
- 页码：555-560
- 全文大小：268 KB
- 参考文献：1. Adams SM (2005) Assessing cause and effect of multiple stressors on marine systems. Mar Pollut Bull 51:649鈥?57 CrossRef
2. Boettcher M, Grund S, Keiter S, Kosmehl T, Reifferscheid G, Seitz N, Rocha PS, Hollert H, Braunbeck T (2010) Comparison of in vitro and in situ genotoxicity in the Danube River by means of the comet assay and the micronucleus test. Mutat Res 700:11鈥?7 CrossRef
3. Ch猫vre N, Gagn茅 F, Blaise C (2003) Development of a biomarker-based index for assessing the ecotoxic potential of aquatic sites. Biomarkers 8:287鈥?98 CrossRef
4. Cotelle S, F茅rard JF (1999) Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 34:246鈥?55 CrossRef
5. de la Torre FR, Salibi谩n A, Ferrari L (2007) Assessment of the pollution impact on biomarkers of effect of a freshwater fish. Chemosphere 68:1582鈥?590 CrossRef
6. Erismis UC, Ci臒erci 陌H, Konuk M (2013) Evaluation of DNA damage in Eurasian marsh frogs ( / Pelophylax ridibundus) by comet assay for determination of possible pollution in the different lakes in central Anatolia, Turkey. Bull Environ Contam Toxicol 90:660鈥?65 CrossRef
7. Frenzilli G, Nigro M, Lyons BP (2009) The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutat Res 681:80鈥?2 CrossRef
8. Hartl MG, Coughlan BM, Sheehan D, Mothersill C, van Pelt FN, O鈥橰eilly SJ, Heffron JJ, O鈥橦alloran J, O鈥橞rien NM (2004) Implications of seasonal priming and reproductive activity on the interpretation of Comet assay data derived from the clam, / Tapes semidecussatus Reeves 1864, exposed to contaminated sediments. Mar Environ Res 57:295鈥?10 CrossRef
9. Koenig S, Porte C, Sol茅 M, Sturve J (2013) Biliary PAH and alkylphenol metabolites, biomarker enzyme activities, and gene expression levels in the deep-sea fish / Alepocephalus rostratus. Environ Sci Technol 47:2854鈥?861 CrossRef
10. Kosmehl T, Hallare AV, Braunbeck T, Hollert H (2008) DNA damage induced by genotoxicants in zebrafish ( / Danio rerio) embryos after contact exposure to freeze-dried sediment and sediment extracts from Laguna Lake (The Philippines) as measured by the comet assay. Mutat Res 650:1鈥?4 CrossRef
11. Lee RF, Steinert S (2003) Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat Res 544:43鈥?4 CrossRef
12. Lu M, Zeng DC, Liao Y, Tong B (2012) Distribution and characterization of organochlorine pesticides and polycyclic aromatic hydrocarbons in surface sediment from Poyang Lake, China. Sci Total Environ 433:491鈥?97 CrossRef
13. McNamee JP, McLean JR, Ferrarotto CL, Bellier PV (2000) Comet assay: rapid processing of multiple samples. Mutat Res 466:63鈥?9 CrossRef
14. Mitchelmore CL, Birmelin C, Livingstone DR, Chipman JK (1998) Detection of DNA strand breaks in isolated mussel ( / Mytilus edulis L.) digestive gland cells using the 鈥淐omet鈥?assay. Ecotoxicol Environ Saf 41:51鈥?8 CrossRef
15. Monteiro V, Cavalcante DGSM, Vil茅la MBFA, Sofia SH, Martinez CBR (2011) In vivo and in vitro exposures for the evaluation of the genotoxic effects of lead on the Neotropical freshwater fish / Prochildus lineatus. Aquat Toxicol 104:291鈥?98 CrossRef
16. Olive PL, Ban谩th JP (2006) The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 1:23鈥?9 CrossRef
17. Rank J, Lehtonen KK, Strand J, Laursen M (2007) DNA damage, acetylcholinesterase activity and lysosomal stability in native and transplanted mussels ( / Mytilus edulis) in areas close to coastal chemical dumping sites in Denmark. Aquat Toxicol 84:50鈥?1 CrossRef
18. Reinecke SA, Reinecke AJ (2004) The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch Environ Contam Toxicol 46:208鈥?15
19. US EPA (2002) Summary of biological assessment programs and biocriteria development for states, tribes, territories, and interstate commissions: streams and wadable river. EPA-822-R-02-048. US EPA, USA
20. Valverde M, Rojas E (2009) Environmental and occupational biomonitoring using the Comet assay. Mutat Res 681:93鈥?09 CrossRef
21. Winter MJ, Day N, Hayes RA, Taylor EW, Butler PJ, Chipman JK (2004) DNA strand breaks and adducts determined in feral and caged chub ( / Leuciscus cephalus) exposed to rivers exhibiting variable water quality around Birmingham, UK. Mutat Res 552:163鈥?75 CrossRef
22. World Health Organization (1995) Environmental Health Criteria 165. International Programme on Chemical Safety, Geneva
23. Wo藕niak K, B艂asiak J (2002) Free radicals-mediated induction of oxidized DNA bases and and DNA-protein cross-links by nickel chloride. Mutat Res 514:233鈥?43 CrossRef - 作者单位：Nami Kang (1)
Han-il Kang (1)
Kwang-Guk An (1)
1. Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of Korea - ISSN：1432-0800
文摘
This study was performed to determine the ecosystem health of an urban stream using DNA damage biomarkers of sentinel fish species along with chemical analyses of water and sediments in the impacted and reference zones. The results based on single-cell gel electrophoresis showed that the extent of DNA damage was significantly elevated in the polluted zone. The severity of DNA damage in the impacted zone was largely attributed to high levels of heavy metals, such as As, Zn, Pb, and Ni. The DNA damage biomarker in benthic fish was sensitive to habitat change, which suggested that this fish species and biomarker are excellent tools for risk assessment. The acute toxicity tests demonstrated that SCGE was sufficient to reflect the different genotypes and pollution statuses and to propose the possibility of zebrafish erythrocytes as competitive materials.