Effects of Instrumental Insemination and Insemination Quantity on Dufour鈥檚 Gland Chemical Profiles and Vitellogenin Expression in Honey Bee Queens (Apis mellifera)

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• 作者：Freddie-Jeanne Richard (1) (2) (3) (4)
  Coby Schal (1) (3)
  David R. Tarpy (1) (3)
  Christina M. Grozinger (1) (2) (3) (5)
  
• 关键词：Chemical ecology ; Gene expression ; Behavior ; Social insects ; Pheromone
• 刊名：Journal of Chemical Ecology
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：37
• 期：9
• 页码：1027-1036
• 全文大小：251KB
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• 作者单位：Freddie-Jeanne Richard (1) (2) (3) (4)
  Coby Schal (1) (3)
  David R. Tarpy (1) (3)
  Christina M. Grozinger (1) (2) (3) (5)
  
  1. Department of Entomology, North Carolina State University, Raleigh, NC, 27695-7613, USA
  2. Department of Genetics, North Carolina State University, Raleigh, NC, 27695-7613, USA
  3. W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC, 27695-7613, USA
  4. Laboratoire Ecologie Evolution Symbiose, UMR CNRS 6556, University of Poitiers, 40 avenue du Recteur Pineau, F-86022, Poitiers, Cedex, France
  5. Department of Entomology, Center for Pollinator Research, Center for Chemical Ecology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, 16802, USA
  

文摘

Honey bee queens (Apis mellifera) mate in their early adult lives with a variable number of males (drones). Mating stimulates dramatic changes in queen behavior, physiology, gene expression, and pheromone production. Here, we used virgin, single drone- (SDI), and multi-drone- (MDI) inseminated queens to study the effects of instrumental insemination and insemination quantity on the pheromone profiles of the Dufour鈥檚 gland, and the expression of the egg-yolk protein, vitellogenin, in the fat body. Age, environmental conditions, and genetic background of the queens were standardized to specifically characterize the effects of these treatments. Our data demonstrate that insemination and insemination quantity significantly affect the chemical profiles of the Dufour鈥檚 gland secretion. Moreover, workers were more attracted to Dufour鈥檚 gland extract from inseminated queens compared to virgins, and to the extract of MDI queens compared to extract of SDI queens. However, while there were differences in the amounts of some esters between MDI queens and the other groups, it appears that the differences in behavioral responses were elicited by subtle changes in the overall chemical profiles rather than dramatic changes in specific individual chemicals. We also found a decrease in vitellogenin gene expression in the fat body of the MDI queens, which is negatively correlated with the quantities of Dufour鈥檚 gland content. The possible explanations of this reduction are discussed.