MALDI-TOF MS meets WGS in a VRE outbreak investigation

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• 作者：S. Schlebusch ; G. R. Price ; R. L. Gallagher…
• 刊名：European Journal of Clinical Microbiology & Infectious Diseases
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：36
• 期：3
• 页码：495-499
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Medical Microbiology; Internal Medicine;
• 出版者：Springer Berlin Heidelberg
• ISSN：1435-4373
• 卷排序：36

文摘

The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.