Traditional and molecular detection methods reveal intense interguild competition and other multitrophic interactions associated with native entomopathogenic nematodes in Swiss tillage soils

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• 作者：Raquel Campos-Herrera (1)
  Geoffrey Jaffuel (1)
  Xavier Chiriboga (1)
  Rub茅n Blanco-P茅rez (1)
  Marie Fesselet (2)
  Vladim铆r P暖啪a (3)
  Fabio Mascher (2)
  Ted C. J. Turlings (1)
  
  1. FARCE Laboratory ; University of Neuch芒tel ; Emile-Argand 11 ; Neuch芒tel ; 2000 ; Switzerland
  2. Agroscope ; Institut of Plant Production Sciences IPS ; Route de Duillier 50 ; CP 1012 ; 1260 ; Nyon ; Switzerland
  3. Laboratory of Entomopathogenic Nematodes ; Institute of Entomology ; Biology Centre ; Czech Academy of Sciences ; Brani拧ovsk谩 31 ; 370 05 ; 膶esk茅 Bud臎jovice ; Czech Republic
  
• 关键词：Entomopathogenic nematodes ; Annual crops ; Soil food web ; Real ; time qPCR ; Galleria mellonella insect ; bait
• 刊名：Plant and Soil
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：389
• 期：1-2
• 页码：237-255
• 全文大小：894 KB
• 参考文献：1. Adams, BJ, Nguyen, KB Taxonomy and systematics. In: Gaugler, R eds. (2002) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, pp. 1-33 CrossRef
  2. Alatorre-Rosas, R, Kaya, HK (1990) Interspecific competition between entomopathogenic nematodes in the genera Heterorhabditis and Steinernema for an insect host in sand. J. Invertebr. Pathol. 55: pp. 179-188 CrossRef
  3. Atkins, SD, Clark, I, Pande, S, Hirsch, PR, Kerry, BR (2005) The use of real-time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiol. Ecol. 51: pp. 257-264 CrossRef
  4. Boemare, N Biology, taxonomy and systematics of Xenorhabdus and Phtorhabdus. In: Gaugler, R eds. (2002) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, pp. 35-56 CrossRef
  5. Campos-Herrera, R, Guti茅rrez, C (2014) Steinernema feltiae intraspecific variability: infection process and sex-ratio. J. Nematol. 46: pp. 35-43
  6. Campos-Herrera, R, Escuer, M, Labrador, S, Robertson, L, Barrios, L, Guti茅rrez, C (2007) Distribution of the entomopathogenic nematodes from La Rioja (Northern Spain). J. Invertebr. Pathol 95: pp. 125-139 CrossRef
  7. Campos-Herrera, R, G贸mez-Ros, JM, Escuer, M, Cuadra, L, Barrios, L, Guti茅rrez, C (2008) Diversity, occurrence, and life characteristics of natural entomopathogenic nematode populations from La Rioja (Northern Spain) under different agricultural management and their relationships with soil factors. Soil Biol. Biochem. 40: pp. 1474-1484 CrossRef
  8. Campos-Herrera, R, El-Borai, FE, Stuart, RJ, Graham, JH, Duncan, LW (2011) Entomopathogenic nematodes, phoretic Paenibacillus spp., and the use of real time quantitative PCR to explore soil food webs in Florida citrus groves. J. Invertebr. Pathol 108: pp. 30-39 CrossRef
  9. Campos-Herrera, R, Johnson, EG, El-Borai, FE, Stuart, RJ, Graham, JH, Duncan, LW (2011) Long-term stability of entomopathogenic nematode spatial patterns measured by sentinel insects and real-time PCR assays. Ann. App. Biol 158: pp. 55-68 CrossRef
  10. Campos-Herrera, R, El-Borai, FE, Larry, LW (2012) Wide interguild relationships among entomopathogenic and free-living nematodes in soil as measured by real time qPCR. J. Invertebr. Pathol. 111: pp. 126-135 CrossRef
  11. Campos-Herrera, R, Ali, JG, D铆az, BM, Duncan, LW (2013) Analyzing spatial patterns linked to the ecology of herbivores and their natural enemies in the soil. Frontiers Plant Sci 4: pp. 1-18
  12. Campos-Herrera, R, Pathak, E, El-Borai, FE, Stuart, RJ, Guti茅rrez, C, Rodr铆guez-Mart铆n, JA, Graham, JH, Duncan, LW (2013) Geospatial patterns of soil properties and the biological control potential of entomopathogenic nematodes in Florida citrus groves. Soil Biol. Biochem. 66: pp. 163-174 CrossRef
  13. Campos-Herrera, R, Pathak, E, El-Borai, FE, Schumann, A, Abd-Elgawad, MMM, Duncan, LW (2013) New citriculture system suppresses native and augmented entomopathogenic nematodes. Biol. Control 66: pp. 183-194 CrossRef
  14. Campos-Herrera, R, El-Borai, FE, Ebert, TA, Schumann, A, Duncan, LW (2014) Management to control citrus greening alters the soil food web and severity of a pest-disease complex. Biol. Control 76: pp. 41-51 CrossRef
  15. Dillman AR, Chaston JM, Adams BJ, Ciche TA, Goodrich-Blair H, Stock SP, Sternberg PW (2012) An entomopathogenic nematode by any other name. PLoS Pathogens 8:e1002527
  16. Dolinski, C, Choo, HY, Duncan, LW (2012) Grower acceptance of entomopathogenic nematodes: case studies on three continents. J. Nematol. 44: pp. 226-235
  17. Duncan, LW, Dunn, DC, Bague, G, Nguyen, K (2003) Competition between entomopathogenic and free-living bactivorous nematodes in larvae of the weevil Diaprepes abbreviatus. J. Nematol. 35: pp. 187-193
  18. Duncan, LW, Graham, JH, Zellers, J, Bright, D, Dunn, DC, El-Borai, FE, Porazinska, DL (2007) Food web responses to augmenting the entomopathogenic nematodes in bare and animal manure-mulched soil. J. Nematol 39: pp. 176-189
  19. Duncan, LW, Stuart, RJ, El-Borai, FE, Campos-Herrera, R, Pathak, E, Graham, JH (2013) Modifying orchard planting sites conserves entomopathogenic nematodes, reduces weevil herbivory and increases citrus tree growth, survival and fruit yield. Biol. Control 64: pp. 26-36 CrossRef
  20. Ekmen, ZI, Hazir, S, Cakmak, I, Ozer, N, Karagoz, M, Kaya, HK (2010) Potential negative effects on biological control by Sancassania polyphyllae (Acari: Acaridae) on an entomopathogenic nematode species. Biol. Control 54: pp. 166-171 CrossRef
  21. El-Borai, FE, Duncan, LW, Preston, JF (2005) Bionomics of a phoretic association between Paenibacillus sp. and the entomopathogenic nematode Steinernema diaprepesi. J. Nematol. 37: pp. 18-25
  22. El-Borai, FE, Brentu, CF, Duncan, LW (2007) Augmenting entomopathogenic nematodes in soil from a Florida citrus orchard: non-target effects of a trophic cascade. J. Nematol. 39: pp. 203-210
  23. Enright, MR, Griffin, CT (2004) Specificity of association between Paenibacillus spp. and the entomopathogenic nematodes, Heterorhabditis spp. Micro. Ecol. 48: pp. 414-423 CrossRef
  24. Enright, MR, McInerney, JO, Griffin, CT (2003) Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. Inter. J. Syst. Evol. Microbiol. 53: pp. 435-441 CrossRef
  25. F茅lix, M-A, Vierstraete, A, Vanfleteren, J (2001) Three biological species closely related to Rhabditis (Oscheius) pseudodolichura K枚rner in Osche, 1952. J. Nematol. 33: pp. 104-109
  26. Freeman, KR, Martin, AP, Karki, D, Lynch, RC, Mitter, MS, Meyer, MS, Longcore, JE, Simmons, DR, Schmidt, SK (2009) Evidence that chytrids dominate fungal communities at high-elevation soils. Proc. Nat. Acad. Sci. 106: pp. 18315-18320 CrossRef
  27. Georgis, R, Koppenh枚fer, AM, Lacey, LA, B茅lair, G, Duncan, LW, Grewal, PS, Samish, M, Tan, L, Torr, P, Tol, RWHM (2006) Successes and failures in the use of parasitic nematodes for pest control. Biol. Control 38: pp. 103-123 CrossRef
  28. Gray, NF Fungi attacking bermiform nematodes. In: Poinar, GO, Jansson, H-B eds. (1988) Diseases of nematodes. CRC Press, Boca Raton, pp. 3-38
  29. Greenwood, CM, Barbercheck, ME, Brownie, C (2011) Short term response of soil microinvertebrates to application of entomopathogenic nematode-infected insects in two tillage systems. Pedobiologia 54: pp. 177-186 CrossRef
  30. Griffin, CT (2012) Perspectives on the behavior of entomopathogenic nematodes from dispersal to reproduction: traits contributing to nematode fitness and biocontrol efficacy. J. Nematol. 44: pp. 177-184
  31. Griffin, CT, O鈥?Callaghan, KM, Dix, I (2001) A self-fertile species of Steinernema from Indonesia: fighter evidence of convergent evolution amongst entomopathogenic nematodes?. Parasitology 122: pp. 181-186 CrossRef
  32. Guti茅rrez, C, Campos-Herrera, R, Jim茅nez, J (2008) Comparative study of selected agrochemical products activity on Steinernema feltiae (Rhabditida: Steinernematidae). Biocontr. Sci. Technol. 18: pp. 101-108 CrossRef
  33. Hominick WM (2002) Biogeography. In: Gaugler R (ed), Entomopathogenic nematology, CABI Publishing, Wallingford, UK, pp: 115鈥?43.
  34. Jaffee, BA, Strong, DR (2005) Strong bottom-up and weak top-down effects in soil: nematode-parasitized insects and nematode-trapping fungi. Soil Biol. Biochem. 37: pp. 1011-1021 CrossRef
  35. Jaffee, BA, Ferris, H, Scow, KM (1998) Nematode-trapping fungi in organic and conventional cropping systems. Pytophatology 88: pp. 344-350 CrossRef
  36. Jenkins, WR (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Dis. Rep. 48: pp. 492
  37. Kaya, HK, Aguillera, MM, Alumai, A, Choo, HY, Torre, M, Fodor, A, Ganguly, S, Hazir, S, Lakatos, T, Pye, A, Wilson, M, Yamanaka, S, Yang, H, Ehlers, R-U (2006) Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biol. Control 38: pp. 134-155 CrossRef
  38. Kondo, E (1989) Studies on the infectivity and propagation of entomogenous nematodes Steinernema spp. (Rhabditida: Steinernematidae) in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Bull. Fac. Agric. Saga Univ 67: pp. 1-87
  39. Kramer I, Hirschy O, Grunder JM (2001)Survey of baited insect parasitic nematodes from the Swiss lowland. In: EUR 19696-COST ACTION 819. Developments in entomopathogenic nematode/bacterial research.Proceedings of the workshop at National University of Ireland Maynooth. 13鈥?5 April 2000.
  40. Larkin, MA, Blackshields, G, Brown, NP, Chenna, R, McGettigan, PA, McWilliam, H, Valentin, F, Wallace, IM, Wilm, A, Lopez, R, Thompson, JD, Gibson, TJ, Higgins, DG (2007) ClustalW2 and ClustalX version 2. Bioinformatics 23: pp. 2947-2948 CrossRef
  41. Lawrence, JL, Hoy, CW, Grewal, PS (2006) Spatial and temporal distribution of endemic entomopathogenic nematodes in a heterogeneous vegetable production landscape. Biol. Control 37: pp. 247-255 CrossRef
  42. Lewis, EE, Campbell, J, Griffin, C, Kaya, HK, Peters, A (2006) Behavioral ecology of entomopathogenic nematodes. Biol. Control 38: pp. 66-79 CrossRef
  43. Liang, W, Zhang, X, Li, Q, Jiang, Y, Ou, W, Neher, DA (2005) Vertical distribution of bacterivorous nematodes under different land uses. J. Nematol. 37: pp. 254-258
  44. Millar, LC, Barbercheck, M (2002) Effects of tillage practices on entomopathogenic nematodes in a corn agroecosystem. Biol. Control 25: pp. 1-11 CrossRef
  45. Mr谩膷ek, Z, P暖啪a, V, Nermut, J (2014) Steinernema poinari sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from the Czech Republic. Zootaxa 3760: pp. 336-350 CrossRef
  46. Nguyen KB (2007) Methodology, morphology and identification. In: Nguyen KB, Hunt DJ (eds.) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts, Nematology Monographs and Perspectives, Vol. 5, Leiden-Boston: Brill, Leiden (The Netherlands), pp: 59鈥?19.
  47. Nguyen KB, Hunt DJ Mra膷ek Z (2007) Steinernematidae: species descriptions. In: Nguyen KB, Hunt DJ (eds.) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives, vol. 5, Leiden-Boston: Brill, Leiden (The Netherlands), pp: 121鈥?09.
  48. Pathak, E, El-Borai, FE, Campos-Herrera, R, Jonhson, EG, Stuart, RJ, Graham, JH, Duncan, LW (2012) Use of real-time PCR to discriminate predatory and saprophagous behavior by nematophagous fungi. Fungal Biol 116: pp. 563-573 CrossRef
  49. Persmark, L, Blank, A, Jansson, H (1996) Population dynamics of nematophagous fungi and nematodes in arable soil: vertical and seasonal fluctuations. Soil Biol. Biochem. 28: pp. 1005-1014 CrossRef
  50. P暖啪a, V, Mr谩膷ek, Z (2009) Mixed infection of Galleria mellonella with two entomopathogenic nematodes (Nematoda: Rhabditida) species: Steinernema affine benefits from the presence of Steinernema kraussei. J. Invertebr. Pathol. 102: pp. 40-43 CrossRef
  51. Rooij-van, D, Goes, PCEM, Putten, WH, Dijk, C (1995) Analysis of nematodes and soil-borne fungi from Ammophila arenaria (Marram grass) in Dutch coastal foredunes by multivariate techniques. Eur. J. Plant Pathol. 101: pp. 149-162 CrossRef
  52. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds.), Bioinformatics methods and protocols: methods in molecular biology. Humana Press. Totowa, (NJ, USA), pp: 365鈥?86.
  53. Steiner, WA (1996) Distribution of entomopathogenic nematodes in the Swiss Alps. Rev. Suisse Zool. 103: pp. 439-452
  54. Stuart, RJ, Barbercheck, ME, Grewal, PS, Taylor, RAJ, Hoy, CW (2006) Population biology of entomopathogenic nematodes: concepts, issues, and models. Biol. Control 38: pp. 80-102 CrossRef
  55. Sturhan, D, Spiridonov, S, Mr谩cek, Z (2005) Steinernema silvaticum sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Europe. Nematology 7: pp. 227-241 CrossRef
  56. Sugar, DR, Murfin, KE, Chaston, JM, Andersen, AW, Richards, GR, L茅on, L, Baum, JA, Clinton, WP, Forst, S, Goldman, BS, Krasomil-Osterfeld, KC, Slater, S, Stock, SP, Goodrich-Blair, H (2012) Phenotypic variation and host interactions of Xenorhabdus bovienii SSe2004, the entomopathogenic symbiont of Steinernema jollieti nematode. Environ. Microbiol. 14: pp. 924-939 CrossRef
  57. Torr, P, Spiridonov, SE, Heritage, S, Wilson, MJ (2007) Habitat associations of two entomopathogenic nematodes: a quantitative study using real-time quantitative polymerase chain reactions. J. Anim. Ecol. 76: pp. 238-245 CrossRef
  58. Ulug, D, Hazir, S, Kaya, HK, Lewis, E (2014) Natural enemies of natural enemies: the potential top-down impact of predators on entomopathogenic nematode populations. Entomological Entomol. 39: pp. 462-469 CrossRef
  59. White, GF (1927) A method for obtaining infective nematode larvae from cultures. Science 66: pp. 302-303 CrossRef
  60. Woodring, J.L., Kaya, H.K., 1988. Steinernematid and Heterorhabditid nematodes: a handbook of techniques. Southern Coop. Ser. Bull., Arkansas Agric. Exp. Sta. Fayetteville, Arkansas.
  61. Zhang, LM, Liu, XZ, Zhu, SF, Chen, SY (2006) Detection of the nematophagous fungus Hirsutella rhossiliensis in soil by real-time PCR and parasitism bioassay. Biol. Control. 36: pp. 316-32 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Soil Science and Conservation
  Plant Physiology
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5036

文摘

Background and aims As part of a research consortium that explores ways to improve soil health, we study how entomopathogenic nematodes (EPNs) can be better exploited for the biological control of soil-dwelling insect pests in annual crops. Methods We evaluated how tillage might affect belowground interactions in two 30-year running Swiss field trials by combining traditional (insect bait) and molecular (novel real-time qPCR protocols) methods. Soil samples (April and October 2013) were evaluated for the presence and activity of EPN soil food web assemblage comprising 13 EPN species, six nematophagous fungi, one ectoparasitic bacterium, and the free-living nematodes (FLN) of the Acrobeloides group. Results Mortality of sentinel larvae, as well as qPCR analyses (for which we provide seven new primers/probes sets) found only trace levels of six EPN species, dominated by heterorhabditids species. Analysis of nematode progeny revealed that EPN compete intensely with FLN for insect cadavers. Overall, it appears that temperate annual cropping systems provide poor environments for EPN and that tillage does not negatively affect the natural occurrence of EPN. Conclusions Natural occurrence of EPN in Swiss tillage soils was very low, and augmentation may be a promising strategy to improve the control of root pests of annual crops.