Direct adventitious shoot regeneration, in vitro flowering, fruiting, secondary metabolite content and antioxidant activity of Scrophularia takesimensis Nakai

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• 作者：Byoung Ryong Jeong ; Iyyakkannu Sivanesan
• 关键词：Endangered plant ; Harpagoside ; Light quality ; HPLC ; Phenolics ; Scrophulariaceae ; Somaclonal variation ; Sucrose
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：123
• 期：3
• 页码：607-618
• 全文大小：986 KB
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• 作者单位：Byoung Ryong Jeong (1)
  Iyyakkannu Sivanesan (1) (2)
  
  1. Institute of Agriculture and Life Science, Department of Horticulture, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660-701, Korea
  2. Department of Molecular Biotechnology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Effects of plant growth regulators, light quality and sucrose on direct adventitious shoot regeneration, in vitro flowering and fruiting of Scrophularia takesimensis were investigated. The highest mean number of shoots per leaf (23.4), petiole (17.2) and stem (20.3) explants was obtained on Murashige and Skoog (MS) medium fortified with 2.0 mg l? BA and 1.0 mg l? IAA under white fluorescent light (WFL). Red light emitting diodes (LED) gave better shoot growth followed by WFL and blue LED. However, red LED treatment decreased the number of roots and induced callus at the base of shoot. The highest number of roots per shoot (9.2) and a maximum root length (8.9 cm) were obtained when the cultures were maintained under WFL. Somaclonal variation was observed when the shoot buds were cultured on the modified MS medium containing 1.0 mg l? IBA. The variant had variegated leaves in comparison with those of normal plantlets. The greatest frequency of flower induction (96.8 %) was obtained when the shoots were cultured on the modified MS medium containing 6.0 % (w/v) sucrose for 45 days under blue LED. The in vitro developed flowers self-fertilized and formed fruits. The culture media and environment had a positive effect on the content of phenolic compounds. Harpagoside content was high in seeds and low in shoots developed in MS medium containing BA. Among the light sources, shoots maintained under blue LED found to be best for harpagoside production (4.9 mg g?) followed by WFL (3.3 mg g?) and red LED (2.7 mg g?). In vitro regenerated shoots had higher capacity to detoxify DPPH free radicals than field-grown plant samples. Keywords Endangered plant Harpagoside Light quality HPLC Phenolics Scrophulariaceae Somaclonal variation Sucrose