A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay
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- 作者：Sebastian Fabritz (1)
Franziska Maa? (1)
Olga Avrutina (1)
Tim Heiseler (1)
Bj?rn Steinmann (1)
Harald Kolmar (1) - 关键词：Alcohol oxidase ; Haloalkane dehalogenase ; Haloalkanes ; Horseradish peroxidase ; Multistage enzyme reaction
- 刊名：AMB Express
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：2
- 期：1
- 全文大小：479KB
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Franziska Maa? (1)
Olga Avrutina (1)
Tim Heiseler (1)
Bj?rn Steinmann (1)
Harald Kolmar (1)
1. Institute of Organic Chemistry and Biochemistry, Technische Universit?t Darmstadt, Petersenstrasse 22, D-64287, Darmstadt, Germany - ISSN：2191-0855
文摘
A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl halides in buffered systems. Irreversible hydrolytic dehalogenation catalyzed by haloalkane dehalogenase DhaA from Rhodococcus erythropolis transfers an alkyl halide into a corresponding alcohol that is further oxidized by alcohol oxidase AOX from Pichia pastoris yielding a respective aldehyde and hydrogen peroxide easily detectable via the horseradish peroxidase catalyzed oxidation of chromogenic molecules. Due to its high sensitivity (0.025?mM, 0.43?ppm for 1,3-dibromopropane), low expenditure and the ability of handling a large number of samples in parallel, this method is an attractive alternative to existing procedures for the monitoring of both haloalkanes and dehalogenases.