Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): induction of triploidy and gynogenesis

详细信息    查看全文

• 作者：Maria Jesús Molina-Luzón ; Jose Ramón López ; Francisca Robles…
• 关键词：Chromosomal manipulation ; Gynogenesis ; Sex ; determination mechanism ; Solea senegalensis ; Triploidy
• 刊名：Journal of Applied Genetics
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：56
• 期：1
• 页码：77-84
• 全文大小：2,215 KB
• 参考文献：1. Arai K (2001) Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture 197:205-28 CrossRef
  2. Baynes SM, Howell BR, Hughes V (2004) Sex-determination in marine flatfish. Extended abstract of a poster presentation in: biotechnologies for quality. Eur Aquac Soc Spec Publ 34(148):149
  3. Bouza C, Hermida M, Pardo BG et al (2007) A microsatellite genetic map of the turbot ( / Scophthalmus maximus). Genetics 177:2457-467
  4. Bouza C, Hermida M, Pardo BG etal (2012) An expressed sequence tag (EST)-enriched genetic map of turbot ( / Scophthalmus maximus): a useful framework for comparative genomics across model and farmed teleosts. BMC Genet 13:54
  5. Cal RM, Gómez J, Castro C etal (2002) Production of haploid and diploid gynogenetic turbot ( / Scophthalmus maximus L.). Spec Publ Eur Aquac Soc 32:179-80
  6. Cal RM, Vidal S, Gómez C etal (2006a) Growth and gonadal development in diploid and triploid turbot ( / Scophthalmus maximus). Aquaculture 251:99-08
  7. Cal RM, Vidal S, Martínez P etal (2006b) Growth and gonadal development of gynogenetic diploid / Scophthalmus maximus. J Fish Biol 68:401-13
  8. Ca?avate JP, Fernández-Díaz C (1999) Influence of co-feeding larvae with live and inert diets on weaning the sole / Solea senegalensis onto commercial dry feeds. Aquaculture 174:255-63 CrossRef
  9. Castro J, Bouza C, Sánchez L etal (2003) Gynogenesis assessment using microsatellite genetic markers in turbot ( / Scophthalmus maximus). Mar Biotechnol 5:584-2
  10. Chen S, Tian Y, Yang J etal (2009) Artificial gynogenesis and sex determination in half-smooth tongue sole ( / Cynoglossus semilaevis). Mar Biotechnol 11:243-51
  11. De la Herrán R, Robles F, Navas JI et al (2008) A highly accurate, single PCR reaction for parentage assignment in Senegal sole based on eight informative microsatellite loci. Aquacult Res 39:1169-174 CrossRef
  12. Don J, Avtalion RR (1988) Production of F1 and F2 diploid gynogenetic tilapias and analysis of the “Hertwig curve-obtained using ultraviolet irradiated sperm. Theor Appl Genet 76:253-59 CrossRef
  13. Felip A, Zanuy S, Carrillo M, Piferrer F (2001) Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species. Genetica 111:175-95 CrossRef
  14. Felip A, Zanuy S, Carrillo M (2006) Comparative analysis of growth performance and sperm motility between precocious and non-precocious males in the European sea bass ( / Dicentrarchus labrax, L.). Aquaculture 256:570-78 CrossRef
  15. Fopp-Bayat D, Jankun M, Woznicki P (2007) Viability of diploid and triploid hybrids of Siberia sturgeon and bester. Aquacult Res 38:1301-304 CrossRef
  16. Funes V, Zuasti E, Catanese G etal (2004) Isolation and characterization of ten microsatellite loci for Senegal sole ( / Solea senegalensis Kaup). Mol Ecol Notes 4:339-41
  17. Garrido-Ramos MA, Jamilena M, Lozano R etal (1994) Cloning and characterization of a fish centromeric satellite DNA. Cytogenet Cell Genet 65:233-37
  18. Haffray P, Lebègue E, Jeu S etal (2009) Genetic determination and temperature effects on turbot scophthalmus maximus sex differentiation: an investigation using steroid sex-inverted males and females. Aquaculture 294:30-6
  19. Havelka M, Hulák M, Ráb P etal (2014) Fertility of a spontaneous hexaploid male Siberian sturgeon, / Acipenser baerii. BMC Genet 15:5
  20. Hendry C, Martin-Robichaud D, Benfey T (2003) Hormonal sex reversal of Atlantic halibut ( / Hippoglossus hippoglossus L.). Aquaculture 219:769-81
  21. Holmefjord I, Refstie T (1997) Induction of triploidy in Atlantic halibut by temperature shocks. Aquacult Int 5:169-73
  22. Howell BR, Baynes SM, Thompson D (1995) Progress towards the identification of the sex determining mechanism of the / Solea (L.) by induction of diploid gynogenesis. Aqualcult Res 26:135-40 CrossRef
  23. Ji X-S, Chen S-L, Liao X-L etal (2009)
• 作者单位：Maria Jesús Molina-Luzón (1)
  Jose Ramón López (2)
  Francisca Robles (1)
  Rafael Navajas-Pérez (1)
  Carmelo Ruiz-Rejón (1)
  Roberto De la Herrán (1)
  Jose Ignacio Navas (2)
  
  1. Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain
  2. IFAPA, Centro Agua del Pino, Consejería de Agricultura y Pesca, Junta de Andalucía, 21450, Cartaya, Huelva, Spain
  
• 刊物主题：Life Sciences, general; Animal Genetics and Genomics; Human Genetics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-3883

文摘

In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems related with sexual maturation, and b) all-female stocks, of higher growth rate. Triploidy was induced by means of a cold shock. Gynogenesis was induced by activating eggs with UV-irradiated sperm, and to prompt diploid gynogenesis, a cold-shock step was also used. Ploidy of putative triploid larvae and gynogenetic embryos were determined by means of karyotyping and microsatellite analysis. Haploid gynogenetic embryos showed the typical “haploid syndrome- As expected, triploid and gynogenetic groups showed lower fertilization, hatching, and survival rates than in the diploid control group. Survival rate, calculated 49?days after hatching, for haploid and diploid gynogenetic groups was similar to those observed in other fish species (0?% and 62.5?%, respectively), whereas triploids showed worse values (45?%). Sex was determined macroscopically and by histological procedures, revealing that all the diploid gynogenetic individuals were females. In conclusion, we have successfully applied chromosomal-manipulation techniques in the flatfish species Senegalese sole in order to produce triploid, haploid, and diploid gynogenetic progenies.