An auto-inducible expression system based on the RhlI-RhlR quorum-sensing regulon for recombinant protein production in E. coli

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• 作者：Rao Ben ; Fan Jiying ; Sun Jian'an…
• 关键词：auto ; inducible expression system ; quorum sensing ; RhlI ; RhlR system
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：21
• 期：1
• 页码：160-168
• 全文大小：462 KB
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• 作者单位：Rao Ben (1)
  Fan Jiying (1)
  Sun Jian’an (1)
  Truong Ngoc Tu (1)
  Sun Jing (1)
  Zhou Jingsong (2)
  Qiuyi (2)
  Shen Yaling (1)
  
  1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200-237, P. R. China
  2. Shanghai Gebaide Biotechnical Co., LTD, Shanghai, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

An artificial network which can accomplish recombinant protein synthesis guided by cell population in E. coli was constructed. The successful functioning of this network requires two plasmids, pWNB and pET. pWNB is responsible for production of T7 RNA polymerase, which controls pET; pET, in turn, regulates the production of target proteins. Several model proteins were tested and the results show that this E. coli system can be used to efficiently express various recombinant proteins. Since system contains T7 RNA polymerase production elements, it is transferable and applicable to well-characterized E. coli strains. Compared to the IPTG-induced system, an equal or greater amount of target protein can be obtained using this auto-inducible expression system in flasks and bioreactors. Our results suggest that it is a competitive alternative to other expression systems used in labs or for industrial applications.