cDNA Clones and Expression Analysis of cpHSC70 and mtHSC70 in Non-Heading Chinese Cabbage

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• 作者：Hongbing Song (1)
  Xiaoming Song (1)
  Huanhuan Liu (1)
  Tongkun Liu (1)
  Ying Li (1)
  Xilin Hou (1)
  
• 关键词：Heat shock protein (HSP) ; Non ; heading Chinese cabbage ; Real ; time PCR ; Sterile line (pol CMS)
• 刊名：Plant Molecular Biology Reporter
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：32
• 期：2
• 页码：531-540
• 全文大小：812 KB
• 作者单位：Hongbing Song (1)
  Xiaoming Song (1)
  Huanhuan Liu (1)
  Tongkun Liu (1)
  Ying Li (1)
  Xilin Hou (1)
  
  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China
  
• ISSN：1572-9818

文摘

The 70-kDa heat shock proteins (HSP70s) are required for thermotolerance and development in plants and other organisms. We applied the techniques of homology-based cloning and real-time quantitative reverse transcription PCR to identify the functions of BccpHSC70s and BcmtHSC70s—heat shock cognates (HSCs) located in chloroplasts and mitochondria, respectively—in non-heading Chinese cabbage (Brassica campestris ssp. chinensis). cDNA clones of BccpHSC70s and BcmtHSC70s were highly homologous to each other and to those from other individual plants. Unlike other HSP70s, BccpHSC70-1 has only two signature motifs (three classical motifs were found in the predicted amino acid sequence of HSP70s, but not all HSP70s contain all three domains), and was expressed constitutively in three detected cultivars; the expression of BccpHSC70-1 in a thermotolerant cultivar (NHCC001) was greater than in other cultivars (NHCC002 and NHCC004) during development. High expression level of BccpHSC70-2 in leaves might be explained by the fact that chloroplasts are sensitive to temperature stress, and this gene might be a candidate gene for developing thermotolerant cultivars of non-heading Chinese cabbage. Expression of BcmtHSC70-2 was inducible under temperature stress. Expression of BcHSC70s in the sterile line showed it played an important role in the process of apoptosis during development of reproductive organs.