Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity
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- 作者：Ruiz Herrera ; José ; Garcí ; a Maceira ; Patricia ; Castillo Barahona ; Luis C. ; Valentí ; n ; Eulogio ; Sentandreu ; Rafael
- 关键词：Calcofluor white ; Lytic enzymes ; Wall mutants ; Wall proteins
- 刊名：Antonie van Leeuwenhoek
- 出版年：2003
- 出版时间：2003
- 年：2003
- 卷：84
- 期：3
- 页码：229-238
- 全文大小：1.85 MB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Medical Microbiology
Plant Sciences
Soil Science and Conservation - 出版者：Springer Netherlands
- ISSN：1572-9699
文摘
A collection of transposon-mutagenized strains of Yarrowia lipolytica was screened for wall defects by determination of their sensitivity to calcofluor white. A number of strains were hypersensitive, whereas others were resistant. Different non-allelic mutants displayed increased sensitivity to autolysis and lytic enzymes, independently of whether they were sensitive or resistant to calcofluor white. A thorough analysis of their cell walls revealed minor quantitative alterations, and no significant changes in chitin content. Electrophoretic analysis of wall-bound and excreted proteins proved to be a sensitive method that revealed defects in the cell wall structure of the mutants. Important alterations in the patterns of the wall proteins extracted by SDS or by enzymatic treatments were noticed for the mutants, as compared to the parental strain. Mutants released to the growth medium a larger number of protein species than the parental strain, suggesting impairment in wall assembly of certain polypeptides. Patterns of wall-bound and excreted proteins, as well as alterations in wall chemical composition were not diagnostic of calcofluor white sensitivity or resistance, but were specific for each mutant. Our data show that an increase in either sensitivity or resistance of Y. lipolytica to certain levels of calcofluor is equally indicative of alterations in cell wall structure, independent of chitin levels.