A bifunctional protease from green alga Ulva pertusa with anticoagulant properties: partial purification and characterization

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• 作者：Seong-Ryeoung Kang ; Jun-Hui Choi ; Dae-Won Kim ; Se-Eun Park…
• 关键词：Anticoagulant ; Antithrombosis ; Bifunctional enzyme ; Fibrinolysis ; Ulva pertusa
• 刊名：Journal of Applied Phycology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：28
• 期：1
• 页码：599-607
• 全文大小：1,709 KB
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• 作者单位：Seong-Ryeoung Kang (1)
  Jun-Hui Choi (2)
  Dae-Won Kim (2)
  Se-Eun Park (3)
  Kumar Sapkota (4)
  Seung Kim (3)
  Sung-Jun Kim (2)
  
  1. Jeonbuk Institute for Bioindustry, Jeonju, 561-360, Republic of Korea
  2. Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju, 501-759, Republic of Korea
  3. Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea
  4. Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  
• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

A bifunctional thrombolytic serine protease (ulvease) having both direct-acting fibrinolytic and plasminogen-activating properties was purified from the green alga, Ulva pertusa. Ulvease had maximum fibrinolytic activity at 40 °C and pH 7.0, and the activity was inhibited by Cu2+ and Zn2+. Fibrinolytic activity of ulvease was higher than that of plasmin and u-PA by fibrin plate assay and displayed both plasmin and plasminogen activator-like activities. Ulvease preferentially hydrolyzed the Aα- and Bβ-chains of fibrinogen, but the γ-chain was appeared unaffected. Ulvease prolonged APTT and PT. PFA-100 assay showed that it delayed the closure times of citrated whole human blood. Ulvease showed a strong thrombolytic ability and safety in vivo, which is better than that of u-PA. It protected mice from collagen- and epinephrine-induced pulmonary thromboembolism. These results suggest that ulvease may have a potential clinical application for the treatment and prevention of thrombosis. Keywords Anticoagulant Antithrombosis Bifunctional enzyme Fibrinolysis Ulva pertusa