Improvements to the HITS-CLIP protocol eliminate widespread mispriming artifacts

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• 作者：Austin E. Gillen ; Tomomi M. Yamamoto ; Enos Kline ; Jay R. Hesselberth…
• 关键词：microRNA ; HITS ; CLIP ; CLIP ; seq ; iCLIP ; PAR ; CLIP
• 刊名：BMC Genomics
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：17
• 期：1
• 全文大小：1242KB
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164
• 卷排序：17

文摘

Background High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) allows for high resolution, genome-wide mapping of RNA-binding proteins. This methodology is frequently used to validate predicted targets of microRNA binding, as well as direct targets of other RNA-binding proteins. Hence, the accuracy and sensitivity of binding site identification is critical.