Genome scaffolding and annotation for the pathogen vector Ixodes ricinus by ultra-long single molecule sequencing
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- 作者：Wibke J. Cramaro ; Oliver E. Hunewald ; Lesley Bell-Sakyi…
- 关键词：Ixodes ricinus ; Genome ; Annotation ; Haploid genome size estimation ; Single molecule real time sequencing ; Flow cytometry ; Tick ; Tick cell line
- 刊名：Parasites & Vectors
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：702KB
- 刊物主题：Parasitology; Entomology; Tropical Medicine; Infectious Diseases; Veterinary Medicine/Veterinary Science; Virology;
- 出版者：BioMed Central
- ISSN：1756-3305
- 卷排序：10
文摘
BackgroundGlobal warming and other ecological changes have facilitated the expansion of Ixodes ricinus tick populations. Ixodes ricinus is the most important carrier of vector-borne pathogens in Europe, transmitting viruses, protozoa and bacteria, in particular Borrelia burgdorferi (sensu lato), the causative agent of Lyme borreliosis, the most prevalent vector-borne disease in humans in the Northern hemisphere. To faster control this disease vector, a better understanding of the I. ricinus tick is necessary. To facilitate such studies, we recently published the first reference genome of this highly prevalent pathogen vector. Here, we further extend these studies by scaffolding and annotating the first reference genome by using ultra-long sequencing reads from third generation single molecule sequencing. In addition, we present the first genome size estimation for I. ricinus ticks and the embryo-derived cell line IRE/CTVM19.