Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein
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- 作者：Shinobu Tsuchiaka ; Sayed Samim Rahpaya ; Konosuke Otomaru ; Hiroshi Aoki…
- 关键词：Bovine enterovirus ; Deep sequencing ; Phylogenetic analysis
- 刊名：BMC Microbiology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：1406KB
- 刊物主题：Microbiology; Biological Microscopy; Mycology; Parasitology; Virology; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-2180
- 卷排序：17
文摘
BackgroundBovine enterovirus (BEV) belongs to the species Enterovirus E or F, genus Enterovirus and family Picornaviridae. Although numerous studies have identified BEVs in the feces of cattle with diarrhea, the pathogenicity of BEVs remains unclear. Previously, we reported the detection of novel kobu-like virus in calf feces, by metagenomics analysis. In the present study, we identified a novel BEV in diarrheal feces collected for that survey. Complete genome sequences were determined by deep sequencing in feces. Secondary RNA structure analysis of the 5′ untranslated region (UTR), phylogenetic tree construction and pairwise identity analysis were conducted.