Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death

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• 作者：Stefan Pittner ; Fabio C. Monticelli…
• 关键词：Postmortem interval (PMI) ; Protein ; Degradation ; Skeletal muscle ; Pig
• 刊名：International Journal of Legal Medicine
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：130
• 期：2
• 页码：421-431
• 全文大小：1,072 KB
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• 作者单位：Stefan Pittner (1)
  Fabio C. Monticelli (2)
  Alexander Pfisterer (1)
  Angela Zissler (1)
  Alexandra M. Sänger (1)
  Walter Stoiber (1)
  Peter Steinbacher (1)
  
  1. Department of Cell Biology, University of Salzburg, Hellbrunnerstr. 34, 5020, Salzburg, Austria
  2. Department of Forensic Medicine and Forensic Neuropsychiatry, University of Salzburg, Ignaz-Harrer-Straße 79, 5020, Salzburg, Austria
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Forensic Medicine
  Medical Law
  Medicine/Public Health, general
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1437-1596

文摘

Estimating the time since death is a very important aspect in forensic sciences which is pursued by a variety of methods. The most precise method to determine the postmortem interval (PMI) is the temperature method which is based on the decrease of the body core temperature from 37 °C. However, this method is only useful in the early postmortem phase (~0–36 h). The aim of the present work is to develop an accurate method for PMI determination beyond this present limit. For this purpose, we used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and casein zymography to analyze the time course of degradation of selected proteins and calpain activity in porcine biceps femoris muscle until 240 h postmortem (hpm). Our results demonstrate that titin, nebulin, desmin, cardiac troponin T, and SERCA1 degraded in a regular and predictable fashion in all samples investigated. Similarly, both the native calpain 1 and calpain 2 bands disintegrate into two bands subsequently. This degradation behavior identifies muscular proteins and enzymes as promising substrates for future molecular-based PMI determination technologies.