Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages

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• 作者：Xin-Ling Wu ; Chian-Jiun Liou ; Zih-Ying Li ; Xuan-Yu Lai…
• 关键词：AMPK ; COX ; 2 ; HO ; 1 ; MAPK ; NF ; κB ; Sesamol
• 刊名：Inflammation Research
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：64
• 期：8
• 页码：577-588
• 全文大小：1,414 KB
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• 作者单位：Xin-Ling Wu (1)
  Chian-Jiun Liou (2)
  Zih-Ying Li (1)
  Xuan-Yu Lai (1)
  Li-Wen Fang (3)
  Wen-Chung Huang (4) (5)
  
  1. Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan
  2. Department of Nursing, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan
  3. Department of Nutrition, I-Shou University, No. 8, Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung, 82445, Taiwan
  4. Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Kwei-Shan, Guishan Dist., Tao-Yuan, 33303, Taiwan
  5. Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Immunology
  Pharmacology and Toxicology
  Allergology
  Dermatology
  Neurology
  Rheumatology
  
• 出版者：Birkh盲user Basel
• ISSN：1420-908X

文摘

Objectives and design Sesamol is a lignan isolated from sesame seed oil. In recent years, it was found that sesamol could decrease lung inflammation and lipopolysaccharide (LPS)-induced lung injury in rats. In this study, we investigated whether sesamol exhibited anti-inflammatory activity in LPS-stimulated macrophages. Materials and methods RAW 264.7 cells were treated with sesamol, then treated with LPS to induce inflammation. The levels of proinflammatory cytokines were analyzed with ELISA. The gene and protein expression of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), and nuclear factor erythroid-2-related factor 2 (Nrf2) were evaluated with real-time PCR and Western blots, respectively. We also examined inflammatory signaling pathways, including nuclear transcription factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. Results Sesamol inhibited production of nitric oxide, prostaglandin E2 (PGE2), and proinflammatory cytokines. Sesamol markedly suppressed mRNA and protein expression of iNOS and COX-2. Sesamol enhanced the protective antioxidant pathway represented by Nrf2 and HO-1. Moreover, sesamol suppressed NF-κB transport into the nucleus and decreased MAPK activation, but it promoted adenosine monophosphate-activated protein kinase (AMPK) activation. Conclusions These data suggested that sesamol ameliorated inflammatory and oxidative damage by upregulating AMPK activation and Nrf2 signaling and blocking the NF-κB and MAPK signaling pathways.