Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds

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• 作者：Min Jeong Kim ; Yong Cheol Shin ; Jong Ho Lee ; Seung Won Jun…
• 关键词：Hydrogel ; 3D scaffolds ; Multiphoton microscopy ; C2C12 myoblast ; Myogenic differentiation
• 刊名：Biomaterials Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：20
• 期：1
• 全文大小：3,225 KB
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• 作者单位：Min Jeong Kim (1)
  Yong Cheol Shin (1)
  Jong Ho Lee (1)
  Seung Won Jun (1)
  Chang-Seok Kim (1)
  Yunki Lee (2)
  Jong-Chul Park (3)
  Soo-Hong Lee (4)
  Ki Dong Park (2)
  Dong-Wook Han (1)
  
  1. Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea
  2. Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Korea
  3. Department of Medical Engineering, Cellbiocontrol Laboratory, Yonsei University College of Medicine, Seoul, 03722, Korea
  4. Department of Biomedical Science, CHA University, Gyeonggi-do, 11160, Korea
  
• 刊物类别：Biomaterials;
• 刊物主题：Biomaterials;
• 出版者：BioMed Central
• ISSN：2055-7124

文摘

Background Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM.