Identification of chromosome regions controlling seed storage proteins of narrow-leafed lupin (Lupinus angustifolius)

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• 作者：Xin Li (1)
  Shahidul Islam (1)
  Huaan Yang (2)
  Wujun Ma (3)
  Guijun Yan (1)
  
• 关键词：Genetic linkage ; Lupinus angustifolius ; MALDI ; TOF ; Narrow ; leafed lupin ; Protein peaks
• 刊名：Journal of Plant Research
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：126
• 期：3
• 页码：395-401
• 全文大小：378KB
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• 作者单位：Xin Li (1)
  Shahidul Islam (1)
  Huaan Yang (2)
  Wujun Ma (3)
  Guijun Yan (1)
  
  1. School of Plant Biology, Faculty of Natural and Agricultural Sciences and Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, WA, 6009, Australia
  2. Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA, 6151, Australia
  3. Molecular Plant Breeding CRC, Centre for Food and Genomic Medicine, State Agriculture Biotechnology Centre, Murdoch University, Western Australian Department of Agriculture and Food, South Street, Perth, WA, 6150, Australia
  
• ISSN：1618-0860

文摘

Narrow-leafed lupin (Lupinus angustifolius L.) is a valuable legume crop for animal feed and human health food because of its high proteins content. However, the genetics of seed storage proteins is unclear, limiting further improvement of protein quantity and quality. In this study, matrix-assisted laser desorption/ionization time of flight mass spectrometry was used for the first time to analyze lupin seed storage proteins and the spectra generated was treated as markers to investigate the chromosome locations controlling seed storage proteins in the narrow-leafed lupin. In a recombinant inbred line population of 89 individuals, 48 polymorphic protein peaks were identified and seven of which were successfully mapped onto four existing linkage groups: two on NLL-04, three on NLL-05, one on NLL-07 and one on NLL-14, with LOD values ranging from 2.6 to 7.7 confirming a significant linkage. Most protein-based markers showed distorted segregation and were failed to be integrated into the reference map. Among them, 31 were grouped into six clusters and the other ten were totally unlinked. This study provides a significant clue to study the comparative genomics/proteomics among legumes as well as for protein marker-assisted breeding. The distribution pattern of genes controlling seed storage protein revealed in this study probably exists universally among legumes or even all plants and animals. Whether genes controlling seed storage protein share the same gene expression pattern controlling other enzymes and what is the mechanism behind it are the questions which remain to be answered in the future.