Elevated mercury bound to serum proteins in methylmercury poisoned rats after selenium treatment

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• 作者：Yunyun Li ; Yuqin Fan ; Jiating Zhao ; Xiaohan Xu ; Hui Jing ; Lihai Shang…
• 关键词：Methylmercury ; Selenite ; Albumin ; SEC–ICP–MS ; MALDI ; TOF ; MS ; Rats
• 刊名：Biometals
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：29
• 期：5
• 页码：893-903
• 全文大小：4,103 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Physical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1572-8773
• 卷排序：29

文摘

Methylmercury is a toxic pollutant and is generated by microbial methylation of elemental or inorganic mercury in the environment. Previous study found decreased hepatic MDA levels and urinary mercury levels in methylmercury poisoned rats after sodium selenite treatment. This study further found increased mercury levels in serum samples from methylmercury poisoned rats after selenium treatment. By using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry, three Hg- binding protein fractions and two Se-binding protein fractions were identified with the molecular weight of approximately 21, 40, and 75 kDa and of 40 and 75 kDa, respectively. Elevated mercury level in the 75 kDa protein fraction was found binding with both Hg and Se, which may explain the decreased urinary Hg excretion in MeHg poisoned rats after Se treatment. MALDI-TOF-MS analysis of the serum found that the 75 kDa protein fractions were albumin binding with both Hg and Se and the 21 kDa fraction was Hg- binding metallothionein.