Characterization and expression analysis of a gene encoding CBF/DREB1 transcription factor from mangrove Aegiceras corniculatum

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• 作者：Ya-Lan Peng ; You-Shao Wang ; Hao Cheng ; Li-Ying Wang
• 关键词：Mangrove plants ; Abiotic stress ; Aegiceras corniculatum ; CBF/DREB1 transcription factor ; Quantitative real ; time PCR
• 刊名：Ecotoxicology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：24
• 期：7-8
• 页码：1733-1743
• 全文大小：1,664 KB
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• 作者单位：Ya-Lan Peng (1)
  You-Shao Wang (1) (2)
  Hao Cheng (1)
  Li-Ying Wang (1)
  
  1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China
  2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

Several transcription factors play important roles in survival of plants under cold, drought and salt stresses by serving as master regulator of sets of downstream stress-responsive genes. A gene encoding CBF/DREB1 transcription factor (C-repeat binding factor/dehydration responsive element-binding factor 1) was isolated from mangrove Aegiceras corniculatum and designated AcCBF1. The full-length cDNA of AcCBF1 was 896 bp containing 618 bp ORF encoding a protein of 205 amino acids. Multiple sequence analysis showed that the corresponding protein had 100 % identity to AmCBF1 (KC776908) from mangrove Avicennia marina, and contains an AP2/ERE DNA-binding domain and two CBF signature sequences. Expression analyses based on quantitative real-time PCR revealed that the AcCBF1 gene was expressed in all tissues of A. corniculatum under normal condition with the highest expression level detected in leaves. When exposed to abiotic stresses, AcCBF1 gene showed different expression patterns in different tissues. Generally, AcCBF1 gene could be rapidly and strongly induced by cold and drought, while slightly induced by abscisic acid and salinity. Furthermore, light could positively regulate the cold-induction level of AcCBF1. These results suggest that the AcCBF1 may be playing important role in the signaling pathway of cold stress and also involved in the cross-talk among abiotic stresses. Further studies focusing on the promotors and downstream stress-responsive genes of AcCBF1 will help to better understand the regulatory mechanisms of mangrove A. corniculatum under abiotic stresses. Keywords Mangrove plants Abiotic stress Aegiceras corniculatum CBF/DREB1 transcription factor Quantitative real-time PCR