In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles

详细信息    查看全文

• 作者：Ying Meng (1)
  Shaoliang Huang (1) (2)
  Jun Min (1) (3)
  Zhongmin Guo (4)
  
• 关键词：ES cell ; differentiation ; hepatocyte ; coagulation factor
• 刊名：Science China Life Sciences
• 出版年：2006
• 出版时间：June 2006
• 年：2006
• 卷：49
• 期：3
• 页码：259-264
• 全文大小：9120KB
• 参考文献：1. Gupta S, Rajvanshi P, Sokhi R P, Vaidya S, Irani A N, Gorla G R. Position-specific gene expression in the liver lobule is directed by the microenvironment and not by the previous cell differentiation state. J Biol Chem, 1999, 274: 2157-165 CrossRef
  2. Grompe M, Laconi E, Shafritz D A. Therapeutic liver repopulation. Semin Liver Dis, 1999, 19: 7-4 CrossRef
  3. Hamazaki T, Iiboshi Y, Oka M, Papst P J, Meacham A M, Zon L I, Terada N. Hepatic maturation in differentiating embryonic stem cells / in vitro. FEBS Lett, 2001, 497: 15-9 CrossRef
  4. Jones E A, Tosh D, Wilson D I, Lindsay S, Forrester L M. Hepatic differentiation of murine embryonic stem cells. Exp Cell Res, 2002, 272: 15-2 CrossRef
  5. Chinzei R, Tanaka Y, Shinmizu-Saito K, Hara Y, Kakinuma S, Watanabe M, Teramoto K, Arii S, Takase K, Sato C, Terada N, Teraoka H. Embryoid-body cells derived from a mouse embryonic stem cell line show differentiation into functional hepatocytes. Hepatology, 2002, 36: 22-9 CrossRef
  6. Choi D, Oh H J Chang U J, Koo S K, Jiang J X, Hwang S Y, Lee J D, Yeoh G C, Shin H S, Lee J S, Oh B. / In vivo differentiation of mouse embryonic stem cells into hepatocytes. Cell Transplant, 2002, 11: 359-68
  7. Shirahashi H, Wu J, Yamamoto N, Catana A, Wege H, Wager B, Okita K, Zern M A. Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. Cell Transplant, 2004, 13: 197-11
  8. Yanmamoto H, Quinn G, Ochiya T, Yamanokuchi H, Teratani T, Terada M, Ochiya T. Differentiation of embryonic stem cells into hepatocytes: Biological functions and therapeutic application. Hepatology, 2003, 37(5): 983-93 CrossRef
  9. Grainger S L, Keeling P W, Brown I M, Marigold J H, Thompson R P H. Clearance and noninvasive determination of the hepatic extraction of indocyanine green in baboons and man. Clin Sci, 1983, 64: 207-12
  10. Yamanaka N, Okamoto E, Keto T, Fujihara S, Sasase S, Oriyama T, Fujimoto J, Furukawa K, Kawamura E, Tomoda F, et al. Usefulness of monitoring the ICG retention rate as an early indicator of allograft function in liver transplantation. Transplant Proc, 1992, 24: 1614-617
  11. Koneru B, Leevy C B, Klein K M, Zweil P. Clearance of indocyanine green in the evaluation of liver donors. Transplantation, 1994, 58: 729-31
  12. Makover A, Soprano D R, Wyatt M L, Goodman D S. An in situ-hybridization study of the localization of retinol-binding protein and transthyretin messenger RNAs during fetal development in the rat. Differentiation, 1989, 40: 17-5
  13. Thomas T, Southwell B R, Schreiber G, Jaworowski A. Plasma protein synthesis and secretion in the visceral yolk sac of the fetal rat: Gene expression, protein synthesis and secretion. Placenta, 1990; 11: 413-30
  14. Pan C J, Lei K J, Chen H, Ward J M, Chou J Y. Ontogeny of the murine glucose-6-phosphatase system. Arch Biochem Biophys, 358: 17-4
  15. Chou J Y. Regulators of fetal liver differentiation / in vitro. Arch Biochem Biophys, 1988, 263: 378-86 CrossRef
  16. Shelly L L, Tynan W, Schmid W, Schutz G, Yeoh G C. Hepatocyte differentiation in vitro: initiation of tyrosine aminotransferase expression in cultured fetal rat hepatocytes. J Cell Biol, 1989, 109: 3403-410 CrossRef
  17. Yamada T, Yoshikawa M, Kanda S, Kato Y, Nakajima Y, Ishizaka S, Tsunoda Y. / In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Stem Cells, 2002, 20: 146-54 CrossRef
  
• 作者单位：Ying Meng (1)
  Shaoliang Huang (1) (2)
  Jun Min (1) (3)
  Zhongmin Guo (4)
  
  1. Center for Stem Cell Research, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China
  2. Department of Pediatrics, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China
  3. Department of Surgery, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China
  4. Experimental Animal Center, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, 510089, China
  

文摘

Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.