Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stress tolerance
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- 作者：Miao Chen (1) (2)
Jingjing Chen (1) (2) (3)
Junyang Fang (1) (2)
Zhenfei Guo (1) (2)
Shaoyun Lu (1) (2) - 关键词：Antioxidants ; Drought tolerance ; Growth ; Rice ; Polyamines ; S ; adenosylmethionine decarboxylase (SAMDC)
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：116
- 期：3
- 页码：311-322
- 全文大小：819 KB
- 作者单位：Miao Chen (1) (2)
Jingjing Chen (1) (2) (3)
Junyang Fang (1) (2)
Zhenfei Guo (1) (2)
Shaoyun Lu (1) (2)
1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
2. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, China
3. South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang, 524091, China - ISSN：1573-5044
文摘
S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme for synthesis of polyamines, regulating plant growth and development as well as plant stress responses. Transgenic rice plants down-regulating OsSAMDC2 were generated by RNA interference. Transcript levels of OsSAMDC2, OsSAMDC1 and OsSAMDC4 were all reduced in transgenic rice, along with decreased levels of sperimidine (Spd) and spermin (Spm) and polyamine oxidase activity. The transgenic lines showed a reduced apical dominance phenotype with lower plant length, higher tiller numbers, reduced and delayed seed germination rate, and decreased pollen viability, seed setting rate and grain yield per plant. Tolerance to multiple abiotic stresses (drought, salinity, and chilling) was also reduced in transgenic plants in association with inhibition of antioxidant enzyme activities under stressed conditions. Our results suggest that Spd and Spm are essential for maintenance of normal plant growth, pollen viability, seed setting rate, grain yield and stress tolerance in rice.