In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis?×?C. dactylon) and their physiological responses to salt and drought stress
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- 作者：Shaoyun Lu ; Xinxiang Peng ; Zhenfei Guo ; Gengyun Zhang ; Zhongcheng Wang ; Congying Wang ; Chaoshu Pang ; Zhen Fan and Jihua Wang
- 关键词：Drought stress ; In vitro selection ; Proline ; Salt stress ; Somaclonal variation ; Triploid bermudagrass
- 刊名：Plant Cell Reports
- 出版年：2007
- 出版时间：August, 2007
- 年：2007
- 卷：26
- 期：8
- 页码：1413-1420
- 全文大小：419 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Plant Sciences
Biotechnology
Plant Biochemistry - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-203X
文摘
A protocol was established for in vitro selection of salinity tolerant somaclonal variations from suspension cultured calli of triploid bermudagrass cv. TifEagle. To induce somaclonal variations the calli were subcultured for 18 months and were then subject to three-round selections for salt-tolerant calli by placing on solid medium containing 0.3 M NaCl for 10 days followed by a recovery for 2 weeks. The surviving calli were regenerated on regeneration medium containing 0.1 M NaCl. Three somaclonal variant lines (2, 71, and 77) were obtained and analyzed. The selected somaclonal lines showed higher relative growth and less injury than TifEagle under salt stress, indicating that they increased salt tolerance. In addition, they had higher relative water content and lower electrolyte leakage than TifEagle after withholding irrigation, indicating that they also increased drought tolerance. The three somaclonal variant lines had higher proline content than TifEagle under normal growth condition. The line 71 had a higher K+/Na+ ratio, whereas the lines 2 and 77 had higher CAT activity under control and salt stress conditions, indicating that different mechanisms for salt tolerance might exist in these three lines.