Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95?°C) and Ringigad (80?°C) hot springs of Uttarakhand, India

详细信息    查看全文

• 作者：Anita Pandey ; Kusum Dhakar ; Avinash Sharma ; Payal Priti…
• 关键词：Thermophilic bacteria ; Hot springs ; Ecological competence
• 刊名：Annals of Microbiology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：65
• 期：2
• 页码：809-816
• 全文大小：221 KB
• 参考文献：Aktuganov G, Melentjev A, Galimzianova N, Khalikova E, Korpela T, Susi P (2008) Wide-range antifungal antagonism of Paenibacillus ehimensis IB-X-b and its dependence on chitinase and beta-1,3-glucanase production. Can J Microbiol 54:577-87View Article PubMed
  Bhardwaj KN, Tiwari SC (2008) Geothermal energy resource utilization: perspectives of the Uttarakhand Himalaya. Curr Sci 95:846-50
  Bhardwaj KN, Tiwari SC, Pandey A (2011) Conservation of thermophilic cyanobacterial diversity and physicochemical characteristics of thermal springs of tapoban geothermal field, Himalayan province (India). Nat Acad Sci Lett 34:77-2
  Bouzas TM, Velázquez JB, Villa TG (2006) Industrial applications of hyperthermophilic enzymes: a review. Protein & Peptide Lett 13:645-51View Article
  Chien A, Edgar DB, Trela JM (1976) Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 127:1550-557PubMed Central PubMed
  Cinti D, Pizzino L, Voltattorni N, Quattrocchi F, Walia V (2009) Geochemistry of thermal waters along fault segments in the beas and parvati valleys (north-west Himalaya, Himachal Pradesh) and in the sohna town (Haryana). Ind Geochem J 43:65-6View Article
  Dhakar K, Jain R, Tamta S, Pandey A (2014) Prolonged laccase production by a cold and pH tolerant strain of Penicillium pinophilum (MCC 1049) isolated from a low temperature environment. Enz Res (Article ID 120708)
  Dwivedi V, Sangwan N, Nigam A, Garg N, Niharika N, Khurana P, Khurana JP, Lal R (2012) Draft genome sequence of Thermus sp. strain RL, isolated from a hot water spring located atop the Himalayan ranges at Manikaran, India. J Bacteriol 194:3534View Article PubMed Central PubMed
  Earl AM, Losick R, Kolter R (2008) Ecology and genomics of Bacillus subtilis. Trends Microbiol 16:269-75View Article PubMed Central PubMed
  Georlette D, Damien B, Blaise V, Depiereux E, Uversky VN, Gerday C, Feller G (2003) Structural and functional adaptations to extreme temperatures in psychrophilic, mesophilic, and thermophilic DNA Ligases. The J Biol Chem 278:37015-7023View Article
  Ghati A, Sarkar K, Paul G (2013) Isolation, characterization and molecular identification of esterolytic thermophilic bacteria from an Indian hot spring. Curr Res Microbiol Biotechnol 1:196-02
  Ghosh D, Bal B, Kashyap VK, Pal S (2003) Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles. Appl Environl Microbiol 69(7):4332-336View Article
  Haki GD, Rakshit SK (2003) Developments in industrially important thermostable enzymes: a review. Biores Technol 89:17-4View Article
  Karatas H, Uyar F, Tolan V, Baysal Z (2013) Optimization and enhanced production of α-amylase and protease by a newly isolated Bacillus licheniformis ZB-05 under solid-state fermentation. Ann Microbiol 63(1):45-2View Article
  Kumar B, Trivedi P, Mishra AK, Pandey A, Palni LMS (2004) Microbial diversity of soil from two hot springs in garhwal Himalaya. Microbiol Res 159:141-46View Article PubMed
  Kumar B, Pandey A, Palni LMS (2005) Extracellular amylase activity of saccharomycopsis fibuligera, a mycelial yeast isolated from a hot spring site in garhwal Himalayas. Ind J Microbiol 45:211-15
  Kumar M, Yadav AN, Tiwari R, Prasanna R, Saxena AK (2013) Deciphering the diversity of culturable thermotolerant bacteria from Manikaran hot springs. Ann Microbiol. doi:10.-007/?s13213-13-709-
  Madslien EH, Olsen JS, Granum PE, Blatny JM (2012) Genotyping of B. licheniformis based on a novel multi-locus sequence typing (MLST) scheme. BMC Microbiol 12:230View Article PubMed Central PubMed
  Marsh CL, Larsen DH (1953) Characterization of some thermophilic bacteria from the hot springs of Yellowstone national park. J Bacteriol 65:193-97PubMed Central PubMed
  Naing KW, Anees M, Kim SJ, Nam Y, Kim YC, Kim KY (2014) Characterization of antifungal activity of Paenibacillus ehimensis KWN38 against soilborne phytopathogenic fungi belonging to various taxonomic groups. Ann Microbiology 64(1):55-3View Article
  Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64(3):548-72View Article PubMed Central PubMed
  Niehaus F, Bertoldo C, Kahler M, Antranikian G (1999) Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol 51:711-29View Article PubMed
  Otto M (2009) Staphylococcus epidermidis- the ‘accidental-pathogen. Nat Rev Microbiol 7:555-67View Article PubMed Central PubMed
  Palmisano MM, Nakamura LK, Duncan KE, Istock CA, Cohan FM (2001) Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the sonoran desert, Arizona. Int J Syst Evol Microbiol 51:1671-679
  Pandey A, Palni LMS (1998) Isolation of Pseudomonas corrugata from Sikkim Himalaya. World J
• 作者单位：Anita Pandey (1)
  Kusum Dhakar (1)
  Avinash Sharma (2)
  Payal Priti (3)
  Priyanka Sati (1)
  Bhavesh Kumar (1) (4)
  
  1. Biotechnological Applications, G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora, 263 643, Uttarakhand, India
  2. Microbial Culture Collection, National Center for Cell Sciences, Ganeshkhind, Pune, 411 007, Maharashtra, India
  3. Department of Microbiology, Barkatullah University, Bhopal, 462 026, Madhya Pradesh, India
  4. Dr. Reddy’s Laboratories Ltd, Proprietary Products R&D, Hyderabad, 500 5090, Andhra Pradesh, India
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Twenty-eight bacterial cultures, isolated from hot springs in Uttarakhand, were characterized with particular reference to their wide temperature and pH tolerance and production of enzymes in the thermophilic range. All the bacterial isolates were observed as Gram-positive or variable rods in varied arrangement. Bacterial isolates exhibited tolerance to a wide temperature range (20-0?°C), from mesophilic (+11° to +45?°C) to thermophilic (+46 ° to +75?°C); few almost reached the hyperthermophilic range (+76?°C). The isolates also tolerated a wide pH range (4-4) and moderate salt concentration. The optimum growth of the bacterial isolates was observed at 55?°C and 7 pH. Out of 28 isolates, 25 produced lipase, 25 amylase, 24 cellulase, 22 protease and 13 xylanase at 55 and 65?°C. Tolerance to a wide temperature and pH range and the production of enzymes in a thermophilic temperature range can be considered as indicators of ecological competence of these bacterial isolates for colonizing the high temperature environment. On the basis of 16S rDNA similarity, 20 bacterial isolates belonged to Bacillus licheniformis, five to Paenibacillus ehimensis and one each to Bacillus sonorensis, B. tequilensis, and Staphylococcus epidermidis. Besides variation in phenotypic characters, strains of B. licheniformis and P. ehimensis showed varying 16S rDNA similarity between 97-9?% and 95-9?%, respectively. Consideration of temperature preferences in classifying microorganisms on the basis of their minimum, maximum, and optimum growth requirements is also discussed. The study has ecological relevance in the context of colonization of high temperature environments by thermophilic bacteria.