Development of gold nanoparticles-based aptasensor for the colorimetric detection of organophosphorus pesticide phorate

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• 作者：Rajni Bala ; Rohit K. Sharma ; Nishima Wangoo
• 关键词：Bioanalytical methods ; Biosensors ; Nanoparticles/nanotechnology
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：408
• 期：1
• 页码：333-338
• 全文大小：1,331 KB
• 参考文献：1.Bala R, Sharma RK, Wangoo N (2015) Highly sensitive colorimetric detection of ethyl parathion using gold nanoprobes. Sens Actuators B 210:425–430CrossRef
  2.Li X, Zhang S, Yu Z, Yang T (2014) Surface-enhanced Raman spectroscopic analysis of phorate and fenthion pesticide in apple skin using silver nanoparticles. Appl Spectrosc 68:483–487CrossRef
  3.Shi Y, Li L, Yang M, Jiang X, Zhao Q, Zhan J (2014) A disordered silver nanowires membrane for extraction and surface-enhanced Raman spectroscopy detection. Analyst 139:2525–2530CrossRef
  4.Pang S, Labuza TP, He L (2014) Development of a single aptamer-based surface enhanced Raman scattering method for rapid detection of multiple pesticides. Analyst 139:1895–1901CrossRef
  5.Zhang C, Wang L, Tu Z, Sun X, He Q, Lei Z, Xu C, Liu Y, Zhang X, Yang J, Liu X, Xu Y (2014) Organophosphorus pesticides detection using broad-specific single-stranded DNA based fluorescence polarization aptamer assay. Biosens Bioelectron 55:216–219CrossRef
  
• 作者单位：Rajni Bala (1)
  Rohit K. Sharma (1)
  Nishima Wangoo (2)
  
  1. Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Sector-14, Chandigarh, 160014, India
  2. Department of Applied Sciences, University Institute of Engineering and Technology (U.I.E.T.), Panjab University, Sector-25, Chandigarh, 160014, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The present study reports a highly simple and rapid method for the detection of a widely used and extremely toxic organophosphorus pesticide, phorate. The detection employs a pesticide-specific aptamer as the recognition element and gold nanoparticles as the optical sensors. The aptamer, owing to its random coil structure, provides stability to the gold nanoparticles upon linking, thereby keeping the nanoparticles well dispersed. However, on the addition of the target pesticide, the aptamer acquires a rigid conformation resulting in the aggregation of the gold nanoparticles. Consequently, the color of the solution changes from red to blue and is easily observable with the naked eye. The proposed method was linear in the concentration range of 0.01 nM to 1.3 μm with the limit of detection as low as 0.01 nM. Moreover, the proposed assay selectively recognized phorate in the presence of other interfering substances and, thus, can be applied to real samples for the rapid and efficient screening of phorate.