Solid-phase microextraction of Methylene Blue using carboxy graphene-modified steel wires, and its detection by electrochemiluminescence

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• 作者：Sui Wang (1)
  Shasha Lv (1)
  Zhiyong Guo (1)
  Feng Jiang (1)
  
• 关键词：Carboxy graphene ; Electrochemiluminescence ; Methylene blue ; Solid ; phase microextraction ; Water samples
• 刊名：Microchimica Acta
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：181
• 期：3-4
• 页码：427-433
• 全文大小：288 KB
• 作者单位：Sui Wang (1)
  Shasha Lv (1)
  Zhiyong Guo (1)
  Feng Jiang (1)
  
  1. Faculty of Materials Science and Chemical Engineering, State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
  
• ISSN：1436-5073

文摘

We report on a new solid phase for microextraction (SPME) of Methylene Blue (MB). It was obtained by immobilizing carboxy graphene (G-COOH) on a stainless steel wire. Scanning electron micrography showed the surface to be homogeneous, porous and wrinkled. The effects of sample solution pH, extraction time, stirring rate, desorption time and of desorption solvent on the efficiency of extraction of MB were optimized. The new SPME was coupled to electrochemiluminescence detection of MB and gave a linear analytical range from 2.7?nM to 1.3?μM, and the detection limit is 0.89?nM which is better than other methods. When considering the enrichment factor of ~20, the resulting detection limit is estimated to be 45?pM. The new SPME fiber was successfully applied to the analysis of MB in spiked real water samples. Recoveries range from 95.7?% to 113.0?%, and relative standard deviations are <5.0?%, which showed the good reproducibility of the method. Figure Scanning electron micrographs of G-COOH fiber coating (A: 10,000× magnification, C: 100× magnification). Laser scanning confocal microscope of G-COOH fiber coating (B, D: a distance of 200?μm)