Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)
详细信息 查看全文
- 作者：Shasha Wang (1)
Fengyang Zhao (2)
Xiaojiao Wei (1)
Bojun Lu (1)
Delin Duan (3)
Gaoge Wang (1) - 关键词：Defense response ; Elicitor ; Female gametophyte ; flg22 ; Saccharina japonica ; Phaeophyta
- 刊名：Journal of Applied Phycology
- 出版年：2013
- 出版时间：August 2013
- 年：2013
- 卷：25
- 期：4
- 页码：1215-1223
- 全文大小：442KB
- 参考文献：1. Baker CJ, Orlandi EW (1995) Active oxygen in plant pathogenesis. Annu Rev Phytopathol 33:299-21 CrossRef
2. Bauer Z, Gómez-Gómez L, Boller T, Felix G (2001) Sensitivity of different ecotypes and mutants of / Arabidopsis thaliana toward the bacterial elicitor flagellin correlates with the presence of receptor-binding sites. J Biol Chem 276:45669-5676 CrossRef
3. Bent AF, Mackey D (2007) Elicitors, effectors, and R genes: the new paradigm and a lifetime supply of questions. Annu Rev Phytopathol 45:399-36 CrossRef
4. Bittel P, Robatzek S (2007) Microbe-associated molecular patterns (MAMPs) probe plant immunity. Curr Opin Plant Biol 10:335-41 CrossRef
5. Bouarab K, Potin P, Correa J, Kloareg B (1999) Sulfated oligosaccharides mediate the interaction between a marine red alga and its green algal pathogenic endophyte. Plant Cell 11:1635-650
6. Che FS, Iwano M, Tanaka N, Takayama S, Minami E, Shibuya N, Kadota I, Isogai A (1999) Biochemical and morphological features of rice cell death induced by / Pseudomonas avenae. Plant Cell Physiol 40:1036-045 CrossRef
7. Che FS, Nakajima Y, Tanaka N, Iwano M, Yoshida T, Takayama S, Kadota I, Isogai A (2000) Flagellin from an incompatible strain of / Pseudomonas avenae induces a resistance response in cultured rice cells. J Biol Chem 275:32347-2356 CrossRef
8. Chinchilla D, Bauer Z, Regenass M, Boller T, Felix G (2006) The / Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. Plant Cell 18:465-76 CrossRef
9. Collén J, Pedersén M, Bornman CH (1994) A stress-induced oxidative burst in / Eucheuma platycladum (Rhodophyta). Plant Physiol 92:417-22 CrossRef
10. Collén J, Rio MJ, García-Reina G, Pedersén M (1995) Photosynthetic production of hydrogen peroxide by / Ulva rigida C. Ag. (Chlorophyta). Planta 196:225-30 CrossRef
11. Felix G, Duran JD, Volko S, Boller T (1999) Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J 18:265-76 CrossRef
12. Gómez-Gómez L, Boller T (2000) FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in / Arabidopsis. Mol Cell 5:1003-011 CrossRef
13. Gómez-Gómez L, Boller T (2002) Flagellin perception: a paradigm for innate immunity. Trends Plant Sci 7:251-56 CrossRef
14. Gómez-Gómez L, Felix G, Boller T (1999) A single locus determines sensitivity to bacterial flagellin in / Arabidopsis thaliana. Plant J 18:277-84 CrossRef
15. Graca JV, Martin MM (1975) Ultrastructural changes in tobacco mosaic virus-induced local lesions in / Nicotiana tabacum L. cv. “Samsu / NN- Physiol Plant Pathol 7:287-91 CrossRef
16. Greenberg JT (1997) Programmed cell death in plant–pathogen interactions. Annu Rev Plant Physiol Plant Mol Biol 48:525-45 CrossRef
17. Heese A, Hann DR, Gimenez-Ibanez S, Jones AME, He K, Li J, Schroeder JI, Peck SC, Rathjen JP (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. PNAS 104:12217-2222 CrossRef
18. Huang J, Tang XX, Liu T, Yan XJ, Jiang M, Li YQ (2002) Alteration of ultrastructures and level of polyphenol and polyphenoloxidase of / Laminaria japonica during alginic acid decomposing bacteria infection. Chinese High Techn Lett 12:74-7 (in Chinese with English abstract)
19. Kunze G, Zipfel C, Robatzek S, Niehaus K, Boller T, Felix G (2004) The N terminus of bacterial elongation factor Tu elicits innate immunity in / Arabidopsis plants. Plant Cell 16:3496-507 CrossRef
20. Küpper FC, Kloareg B, Guern J, Potin P (2001) Oligoguluronates elicit an oxidative burst in the brown algal kelp / Laminaria digitata. Plant Physiol 125:278-91 CrossRef
21. Kupper FC, Gaquerel E, Boneberg EM, Morath S, Salaun JP, Potin P (2006) Early events in the perception of lipopolysaccharides in the brown alga / Laminaria digitata include an oxidative burst and activation of fatty acid oxidation cascades. J Exp Bot 57:1991-999 CrossRef
22. Leblanc C, Colin C, Cosse A, Delage L, La Barre S, Morin P, Fiévet B, Voiseux C, Ambroise Y, Verhaeghe E, Amouroux D, Donard O, Tessier E, Potin P (2006) Iodine transfers in the coastal marine environment: the key role of brown algae and of their vanadium-dependent haloperoxidases. Biochimie 88:1773-785 CrossRef
23. Marja PK, Anu IH, Heikki JV, Jussi-Pekka R, Kalevi P, Tytti SK, Marina H (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954-962 CrossRef
24. Meindl T, Boller T, Felix G (2000) The bacterial elicitor flagellin activates its receptor in tomato cells according to the address-message concept. Plant Cell 12:1783-794
25. Menéndez-Benavente L, Teixeira FK, Kamei CLA, Pinheiro MM (2004) Salt stress induces expression of genes encoding antioxidant enzymes in seedlings of a Brazilian indica rice ( / Oryza sativa L.). Plant Sci 166:323-31 CrossRef
26. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405-10 CrossRef
27. Mittler R, Lam E (1995) Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death. Plant Cell 7:1951-962
28. Mittler R, Simon L, Lam E (1997) Pathogen-induced programmed cell death in tobacco. J Cell Sci 110:1333-344
29. Mizuta H, Yasui H (2010) Significance of radical oxygen production in sorus development and zoospore germination in / Saccharina japonica (Phaeophyceae). Bot Mar 53:409-16 CrossRef
30. Naito K, Taguchi F, Suzuki T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y (2008) Amino acid sequence of bacterial microbe-associated molecular pattern flg22 is required for virulence. Mol Plant Microbe Interact 21:1165-174 CrossRef
31. Navarro L, Bari R, Achard P, Lisón P, Nemri A, Harberd NP, Jones JD (2008) DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling. Curr Biol 18(9):650-55 CrossRef
32. Nürnberger T, Brunner F, Kemmerling B, Piater L (2004) Innate immunity in plants and animals: striking similarities and obvious differences. Immunol Rev 198:249-66 CrossRef
33. Peng M, Kùc J (1992) Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathology 82:696-99 CrossRef
34. Pennell RI, Lamb C (1997) Programmed cell death in plants. Plant Cell 9:1157-168 CrossRef
35. Postel S, Kemmerling B (2009) Plant systems for recognition of pathogen-associated molecular patterns. Semin Cell Dev Biol 20:1025-031 CrossRef
36. Roebuck P, Sexton R, Mansfield JW (1978) Ultrastructural observations on the development of the hypersensitive reaction in leaves of / Phaseolus vulgaris cv. Red Mexican inoculated with / Pseudomonas phaseolicola (race 1). Physiol Plant Pathol 12:151-57 CrossRef
37. Sánchez-Moreno C, Larrauri JA, Saura-Calixto F (1999) Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res Int 32:407-12 CrossRef
38. Sasabe M, Takeuchi K, Kamoun S, Ichinose Y, Govers F, Toyoda K, Shiraishi T, Yamada T (2000) Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture. Eur J Biochem 267:5005-013 CrossRef
39. Schuster SC, Khan S (1994) The bacterial flagellar motor. Annu Rev Biophys Biomol Struct 23:509-39 CrossRef
40. Shahidi F, Wanasundara PKJPD (1992) Phenolic antioxidants: criteria review. Food Sci Nutr 32:67-03
41. Strack D (1997) Phenolic metabolism. In: Dey PM, Harborne JB (eds) Plant Biochemistry. Academic Press, New York, pp 387-16 CrossRef
42. Sugihara N, Arakawa T, Ohnishi M, Furuno K (1999) Anti and pro-oxidative effects of flavonoids on metal induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes located with α-linolenic acid. Free Radic Biol Med 27:1313-323 CrossRef
43. Tang XX, Yang Z, Huang J, Yan XJ (2001) Effects of infection by algnic acid decomposing bacteria on activity of antioxidant enzymes in / Laminaria japonica. J Fish Sci China 25:424-27 (in Chinese with English abstract)
44. Van Breusegem F, Dat JF (2006) Reactive oxygen species in plant cell death. Plant Physiol 141:384-90 CrossRef
45. Wang GG (2003) Studies on defense mechanisms of / Laminaria to pathogens. Dissertation, Institute of Oceanology, Chinese Academy of Sciences, Qingdao
46. Wang GG, Liu XY, Li XH, Lin W, Yan XJ, Duan DL (2004a) Spatial-temporal changes of Ca2+ distribution in cells of / Laminaria japonica infected by alginic acid degradation bacteria. Chin J Oceanol Limnol 9:36
47. Wang GG, Lin W, Zhang LJ, Yan XJ, Duan DL (2004b) Programmed cell death in / Laminaria japonica (Phaeophyta) tissues infected with alginic acid decomposing bacterium. Prog Nat Sci 14:1064-068 CrossRef
48. Wang GG, Shuai L, Li Y, Lin W, Zhao XW, Duan DL (2008) Phylogenetic analysis of epiphytic marine bacteria on the hole-rotten diseased sporophytes of / Laminaria japonica. J Appl Phycol 20:403-09 CrossRef
49. Wang SS, Wei XJ, Lu BJ, Wang GG (2012) Preliminary study on flg22-induced defense responses in sporophytes of / Saccharina japonica (Phaeophyta). J Fish China (in Chinese with English abstract, in press)
50. Weinberger F (2007) Pathogen-induced defense and innate immunity in macroalgae. Biol Bull 213:290-02 CrossRef
51. Weinberger F, Friedlander M (2000) Response of / Gracilaria conferta (Rhodophyta) to oligoagars results in defense against agar-degrading epiphytes. J Phycol 36:1079-086 CrossRef
52. Weinberger F, Friedlander M, Hoppe HG (1999) Oligoagars elicit a physiological response in / Gracilaria conferta (Rhodophyta). J Phycol 35:747-55 CrossRef
53. Weinberger F, Richard C, Kloareg B, Kashman Y, Hoppe HG, Friedlander M (2001) Structure-activity relationships of oligoagar elicitors toward / Gracilaria conferta (Rhodophyta). J Phycol 37:418-26 CrossRef
54. Zhang WW (2003) Histochemical changes and occurrence of phytoalexins in / Laminaria japonica during host’s anti-pathogenesis process. Dissertation, Institute of Oceanology, Chinese Academy of Sciences, Qingdao
55. Zipfel C (2008) Pattern-recognition receptors in plant innate immunity. Immunol Rev 20:10-6
56. Zipfel C (2009) Early molecular events in PAMP-triggered immunity. Plant Biol 12:414-20
57. Zipfel C, Robatzek S, Navarro L, Oakeley EJ, Jones JD, Felix G, Boller T (2004) Bacterial disease resistance in arabidopsis through flagellin perception. Nature 428:764-67 CrossRef - 作者单位：Shasha Wang (1)
Fengyang Zhao (2)
Xiaojiao Wei (1)
Bojun Lu (1)
Delin Duan (3)
Gaoge Wang (1)
1. College of Marine Life Sciences, Ocean University of China, Yushan Road No. 5, Qingdao, 266003, People’s Republic of China
2. Qingdao No. 39 Middle School, Dengzhou Road No. 5, Qingdao, 266003, People’s Republic of China
3. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road No. 7, Qingdao, 266071, People’s Republic of China
文摘
Flg22, which is the most conserved 22-amino acid peptide in the N-terminal part of flagellin, functions as an effective elicitor in higher plants. Marine algae and higher plants share some conserved characteristics on defense response pathways. Flg22-induced defense responses were investigated in female gametophytes of Saccharina japonica. Condensation of chloroplasts and thickened cell walls, as well as relatively stable structures of mitochondria and nucleus indicated that there were hypersensitive programmed cell death occurred after induction by flg22. By using luminol-dependent luminescence detection method, rapid release of H2O2 was detected in the induced female gametophytes and reached a peak concentration of about 46?μM at 2?h. Reactive oxygen species production was also observed histologically using the fluorescent dye 2-7-dichlorofluorescein diacetate, showing a consistent result with quantitative analysis of H2O2. Furthermore, results of antioxidant enzyme activities indicated that there was a trend in the order of catalase > superoxide dismutase > glutathione peroxidase. Finally, high level phenol content of cell-free extracts was found after flg22 induction. According to our results, flg22 could be an effective elicitor which could induce defense responses in female gametophytes of S. japonica.