Keratinocytes derived from chicken embryonic stem cells support Marek’s disease virus infection: a highly differentiated cell model to study viral replication and morphogenesis

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• 作者：Mathilde Couteaudier ; Katia Courvoisier ; Laetitia Trapp-Fragnet…
• 关键词：Marek’s disease virus ; Chicken keratinocytes ; Viral replication ; Viral morphogenesis
• 刊名：Virology Journal
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：13
• 期：1
• 全文大小：3,902 KB
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• 作者单位：Mathilde Couteaudier (1)
  Katia Courvoisier (1)
  Laetitia Trapp-Fragnet (1)
  Caroline Denesvre (1)
  Jean-François Vautherot (1)
  
  1. INRA — Université François-Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, ISP, F-37380, Nouzilly, France
  
• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X

文摘

Background Marek’s disease is a virus disease with worldwide distribution that causes major losses to poultry production. Vaccines against Marek’s disease virus, an oncogenic alphaherpesvirus, reduce tumour formation but have no effect on virus shedding. Successful horizontal virus transmission is linked to the active viral replication in feather follicle epithelial cells of infected chickens, from which infectious viral particles are shed into the environment. The feather follicle epithelium is the sole tissue in which those infectious particles are produced and no in vitro cell-systems can support this highly efficient morphogenesis. We previously characterized embryonic stem-cell-derived keratinocytes, showing they display a marker-gene profile similar to skin keratinocytes, and therefore we tested their susceptibility to Marek’s disease virus infection.