The Influence of Glyphosate on the Microbiota and Production of Botulinum Neurotoxin During Ruminal Fermentation

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• 作者：Wagis Ackermann ; Manfred Coenen ; Wieland Schr?dl ; Awad A. Shehata…
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：70
• 期：3
• 页码：374-382
• 全文大小：910 KB
• 参考文献：1. Abou Akkada AR, Howard BH (1960) The biochemistry of rumen protozoa. 3. The carbohydrate metabolism of / Entodinium. Biochem J 76:445-51
  2. Adlerberth I, Cerquetti M, Poilane I, Wold A, Collignon A (2000) Mechanisms of colonisation and colonisationn resistance of the digestive tract Part 1: bacteria: host interactions. Microb Ecol Health Dis 2:223-39 CrossRef
  3. Allison M, Maloy S, Matson R (1976) Inactivation of / Clostridium botulinum toxin by ruminal microbes from cattle and sheep. Appl Environ Microbiol 32:685-88
  4. Arregui MC, Lenardón A, Sanchez D, Maitre MI, Scotta R, Enrique S (2004) Monitoring glyphosate residues in transgenic glyphosate-resistant soybean. Pest Manag Sci 60:163-66 CrossRef
  5. Benachour N, Sipahutar H, Moslemi S, Gasnier C, Travert C, Séralini GE (2007) Time-and dose-dependent effects of roundup on human embryonic and placental cells. Arch Environ Contam Toxicol 53:126-33 CrossRef
  6. Benachour N, Séralini GE (2009) Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chem Res Toxicol 22:97-05 CrossRef
  7. B?hnel H, Schwagerick B, Gessler F (2001) Visceral botulism—a new form of bovine / Clostridium botulinum toxication. J Vet Med 48:373-83 CrossRef
  8. Burggraf S, Mayer T, Amann R, Schadhauser S, Woese CR, Stetter KO (1994) Identifying members of the domain Archaea with rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 60:3112-119
  9. Cakmak I, Yazici A, Tutus Y, Ozturk L (2009) Glyphosate reduced seed and leaf concentrations of calcium, manganese, magnesium, and iron in non-glyphosate resistant soybean. Eur J Agron 31:114-19 CrossRef
  10. Coleman GS (1985) The cellulase content of 15 species of entodiniomorphid protozoa, mixed bacteria and plant debris isolated from the bovine rumen. J Agric Sci 104:349 CrossRef
  11. Coleman GS (1986) The amylase activity of 14 species of entodiniomorphid protozoa and the distribution of amylase in rumen digesta fractions of sheep containing no protozoa or one of seven different protozoal populations. J Agric Sci 107:709 CrossRef
  12. Coleman G (1992) The rate of uptake and metabolism of starch grains and cellulose particles by / Entodinium species, / Eudiplodinium maggii some other entodiniomorphid protozoa and natural protozoal populations taken from the bovine rumen. J Appl Bacteriol 73:507-13 CrossRef
  13. Cringoli G, Rinaldi L, Veneziano V, Capelli G, Scala A (2004) The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and / Dicrocoelium dendriticum in sheep. Vet Parasitol 123:121-31 CrossRef
  14. Curwin BD, Hein MJ, Sanderson WT, Striley C, Heederik D et al (2007) Urinary pesticide concentrations among children, mothers and fathers living in farm and non-farm households in iowa. Ann Occup Hyg 51:53-6 CrossRef
  15. Dahlenborg M, Borch E, Radstr?m P (2003) Prevalence of / Clostridium botulinum types B, E and F in faecal samples from Swedish cattle. Int J Food Microbiol 82:105-10 CrossRef
  16. Demeyer D (1981) Rumen microbes and digestion of plant cell walls. Agric Environ 6:295-37 CrossRef
  17. Duke SO, Powles SB (2008) Glyphosate: a once-in-a-century herbicide. Pest Manag Sci 64:319
• 作者单位：Wagis Ackermann (1)
  Manfred Coenen (2)
  Wieland Schr?dl (1)
  Awad A. Shehata (1) (3)
  Monika Krüger (1)
  
  1. Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103, Leipzig, Saxony, Germany
  2. Institute of Animal Nutrition, Nutrition Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Saxony, Germany
  3. Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Sadat City University, Sadat City, Egypt
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

The aim of the present study is to investigate the impact of glyphosate on the microbiota and on the botulinum neurotoxin (BoNT) expression during in vitro ruminal fermentation. This study was conducted using two DAISYII-incubators with four ventilated incubation vessels filled with rumen fluid of a 4-year-old non-lactating Holstein–Friesian cow. Two hundred milliliter rumen fluid and 800?ml buffer solution were used with six filter bags containing 500?mg concentrated feed or crude fiber-enriched diet. Final concentrations of 0, 1, 10, and 100?μg/ml of glyphosate in the diluted rumen fluids were added and incubated under CO2-aerated conditions for 48?h. The protozoal population was analyzed microscopically and the ruminal flora was characterized using the fluorescence in situ hybridization technique. Clostridium botulinum and BoNT were quantified using most probable number and ELISA, respectively. Results showed that glyphosate had an inhibitory effect on select groups of the ruminal microbiota, but increased the population of pathogenic species. The BoNT was produced during incubation when inoculum was treated with high doses of glyphosate. In conclusion, glyphosate causes dysbiosis which favors the production of BoNT in the rumen. The global regulations restrictions for the use of glyphosate should be re-evaluated.