Development and characterization of 17 microsatellite markers for Chinese soft-shelled turtle, Pelodiscus sinensis

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• 作者：Xiao Ma (1) (2)
  Gang Xiong (1) (3)
  Luming Wang (1)
  Luqi Yan (1)
  Xuejun Li (2)
  Dizhi Xie (2)
  Xiaoqing Wang (1)
  
• 关键词：Pelodiscus sinensis ; Chinese soft ; shelled turtle ; Microsatellite ; Next generation sequencing
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：4
• 页码：955-956
• 全文大小：148 KB
• 参考文献：1. Fritz U, Gong S, Auer M, Kuchling G, Schneewei? N, Hundsd?rfer AK (2010) The world’s economically most important chelonians represent a diverse species complex (Testudines: Trionychidae: Pelodiscus). Org Divers Evol 10:227-42 CrossRef
  2. Kalinowski S, Taper M, Marshall T (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol Notes 16:1099-106 CrossRef
  3. Que Y, Zhu B, Rosenthal H, Chang J (2007) Isolation and characterization of microsatellites in Chinese soft-shelled turtle, / Pelodiscus sinensis. Mol Ecol Notes 7:1265-267 CrossRef
  4. Yeh FC, Boyle TJB (1997) Population genetic analysis of codominant and dominant markers and quantitative traits. Belg J Bot 129:157
  
• 作者单位：Xiao Ma (1) (2)
  Gang Xiong (1) (3)
  Luming Wang (1)
  Luqi Yan (1)
  Xuejun Li (2)
  Dizhi Xie (2)
  Xiaoqing Wang (1)
  
  1. College of Animal Science and Technology, Hunan Agriculture University, Changsha, 410128, Hunan, China
  2. College of Fisheries, Henan Normal University, Xinxiang, 453007, Henan, China
  3. Hunan Biological Electromechanical Vocational and Technical College, Changsha, 410127, Hunan, China
  
• ISSN：1877-7260

文摘

A set of 17 polymorphic microsatellite markers were isolated and characterized for the Chinese soft-shelled turtle (Pelodiscus sinensis) using a next generation sequencing approach. The number of alleles per locus ranged from 2 to 11. The observed, expected heterozygosities and polymorphic information content per locus varied from 0.103 to 0.750, 0.222 to 0.883 and 0.158 to 0.788, respectively. Significant linkage disequilibrium and deviations from the Hardy–Weinberg disequilibrium between two pairs of loci were found. These loci will be beneficial for the population genetic structure investigation and marker assisted breeding for P. sinensis.