Rice choline monooxygenase (OsCMO) protein functions in enhancing glycine betaine biosynthesis in transgenic tobacco but does not accumulate in rice (Oryza sativa L. ssp. japonica)

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• 作者：Di Luo (1)
  Xiangli Niu (2)
  Jinde Yu (1)
  Jun Yan (3)
  Xiaojun Gou (3)
  Bao-Rong Lu (4) brlu@fudan.edu.cn
  Yongsheng Liu (12) liuyongsheng1122@yahoo.com.cn
• 关键词：Oryza sativa L. R11 ; Glycine betaine R11 ; Choline monooxygenase R11 ; Pseudogene R11 ; Salt stress
• 刊名：Plant Cell Reports
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：31
• 期：9
• 页码：1625-1635
• 全文大小：853.5 KB
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• 作者单位：1. Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Science and State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064 China2. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009 China3. Laboratory for Chemistry of Traditional Chinese medicine, Chengdu University, Chengdu, 610106 China4. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai, 200433 China
• ISSN：1432-203X

文摘

Glycine betaine (GB) is a compatible quaternary amine that enables plants to tolerate abiotic stresses, including salt, drought and cold. In plants, GB is synthesized through two-step of successive oxidations from choline, catalyzed by choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), respectively. Rice is considered as a typical non-GB accumulating species, although the entire genome sequencing revealed rice contains orthologs of both CMO and BADH. Several studies unraveled that rice has a functional BADH gene, but whether rice CMO gene (OsCMO) is functional or a pseudogene remains to be elucidated. In the present study, we report the functional characterization of rice CMO gene. The OsCMO gene was isolated from rice cv. Nipponbare (Oryza sativa L. ssp. japonica) using RT-PCR. Northern blot demonstrated the transcription of OsCMO is enhanced by salt stress. Transgenic tobacco plants overexpressing OsCMO results in increased GB content and elevated tolerance to salt stress. Immunoblotting analysis demonstrates that a functional OsCMO protein with correct size was present in transgenic tobacco but rarely accumulated in wild-type rice plants. Surprisingly, a large amount of truncated proteins derived from OsCMO was induced in the rice seedlings in response to salt stresses. This suggests that it is the lack of a functional OsCMO protein that presumably results in non-GB accumulation in the tested rice plant.