The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.

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• 作者：Xue-Chao Hu ; Lu-Jing Ren ; Sheng-Lan Chen ; Li Zhang&#8230
• 关键词：Schizochytrium sp. ; Osmotic pressure ; Docosahexaenoic acid ; Salts
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：38
• 期：11
• 页码：2129-2136
• 全文大小：848 KB
• 参考文献：1.Horrocks LA, Yeo YK (1999) Health benefits of docohexaenoic acid (DHA). Pharmacol Res 40:211鈥?25CrossRef
  2.Rubio-Rodr铆guez N, Beltr谩n S, Jaime I, Diego SM, Sanz MT, Carballido JR (2010) Production of omega-3 polyunsaturated fatty acid concentrates: a review. Innov Food Sci Emerg. 11:1鈥?2CrossRef
  3.Yokochi T, Honda D, Higashihara T, Nakahara T (1998) Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. Appl Microbiol Biotechnol 49:72鈥?6CrossRef
  4.Lang F, Busch G, Ritter M, Volkl H, Waldegger S, Gulbins E, Haussinger D (1998) Functional significance of cell volume regulatory mechanisms. Physiol Rev 78:247鈥?06
  5.Hohmann S (2002) Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol Mol Biol R. 66:300鈥?72CrossRef
  6.Liu L, Xu Q, Li Y, Shi Z, Zhu Y, Du G, Chen J (2007) Enhancement of pyruvate production by osmotic-tolerant mutant of Torulopsis glabrata. Biotechnol Bioeng 97:825鈥?32CrossRef
  7.Yang LB, Zhan XB, Zheng ZY, Wu JR, Gao MJ, Lin CC (2014) A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol. Bioresour Technol 151:120鈥?27CrossRef
  8.Takagi M, Karseno Yoshida T (2006) Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. J Biosci Bioeng 101:223鈥?26CrossRef
  9.Ren LJ, Ji XJ, Huang H, Qu L, Feng Y, Tong QQ, Ouyang PK (2010) Development of a stepwise aeration control strategy for efficient docosahexaenoic acid production by Schizochytrium sp. Appl Microbiol Biotechnol 87:1649鈥?656CrossRef
  10.Qu L, Ji XJ, Nie Ren LJ, Feng ZK, Wu WJ, Ouyang PK, Huang H (2011) Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply control strategy based on oxygen transfer coefficient. Lett Appl Microbiol 52:22鈥?7CrossRef
  11.Zeng Y, Ji XJ, Lian M, Ren LJ, Jin LJ, Ouyang PK, Huang H (2011) Development of a temperature shift strategy for efficient docosahexaenoic acid production by a marine fungoid protist, Schizochytrium sp. HX-308. Appl Biochem Biotechnol 164:249鈥?55CrossRef
  12.Li X, Lin Y, Chang M, Jin Q, Wang X (2015) Efficient production of arachidonic acid by Mortierella alpina through integrating fed-batch culture with a two-stage pH control strategy. Bioresour Technol 181:275鈥?82CrossRef
  13.Akimoto M, Ishii T, Yamagaki K, Ohtaguchi K, Koide K, Yazawa K (1991) Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from Macherel intestines. J Am Oil Chem Soc 68:504鈥?08CrossRef
  14.Min KH, Lee HH, Anbu P, Chaulagain BP, Hur BK (2012) The effects of culture condition on the growth property and docosahexaenoic acid production from Thraustochytrium aureum ATCC 34304. Korean J Chem Eng 29:1211鈥?215CrossRef
  15.Ren LJ, Huang H, Xiao AH, Lian M, Jin LJ, Ji XJ (2009) Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308. Bioprocess Biosyst Eng 32:837鈥?43CrossRef
  16.Sun LN, Zhuang LJ, Yi X, Ji XJ, Yan JC, Huang H (2014) Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp. Bioresour Technol 159:199鈥?06CrossRef
  17.Ren LJ, Sun GN, Ji XJ, Hu XC, Huang H (2014) Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp. Bioresour Technol 157:107鈥?13CrossRef
  18.Wright PJ, Reed RH (1988) Method for determination of turgor pressure in macroalgae, with particular reference to the Phaeophyta. Mar Biol 99(4):473鈥?80CrossRef
  19.Ling XP, Guo J, Liu X, Zhang X, Wang N, Lu Y, Ng IS (2015) Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp LU310. Bioresour Technol 184:139鈥?47CrossRef
  20.Ren LJ, Sun LN, Zhuang XY, Qu L, Ji XJ, Huang H (2014) Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition. Bioprocess Biosyst Eng 37:865鈥?72CrossRef
  21.Ren LJ, Feng Y, Li J, Qu L, Huang H (2013) Impact of phosphate concentration on docosahexaenoic acid production and related enzyme activities in fermentation of Schizochytrium sp. Bioprocess Biosyst Eng 36:1177鈥?183CrossRef
  22.Yan L, Bogle IDL (2007) A visualization method for operating optimization. Comput Chem Eng 31:808鈥?14CrossRef
  23.Liu Y, Tang J, Li JJ, Daroch M, Cheng HH (2015) Efficient production of triacylglycerols rich in docosahexaenoic acid (DHA) by osmo-heterotrophic marine protists. Appl Microbiol Biotechnol 98:9643鈥?652CrossRef
  24.Tashima Y, Yoshimura N (1975) Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions. J Biochem 78:1161鈥?169
  25.Lorimer GH, Badger MR, Andrews TJ (1976) The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry 15:529鈥?36CrossRef
  26.Kumar S, Gummadi SN (2009) Osmotic adaptation in halotolerant yeast, Debaryomyces nepalensis NCYC 3413: role of osmolytes and cation transport. Extremophiles 13:793鈥?05CrossRef
  27.Flahaut S, Benachour A, Giard JC, Boutibonnes P, Auffray Y (1996) Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433. Arch Microbiol 165:317鈥?24CrossRef
  28.Lerudulier D, Strom AR, Dandekar AM, Smith AT, Valentine RC (1984) Moleeular biology of osmoregulation. Science 224:1064鈥?068CrossRef
  29.Li J, Liu RJ, Chang GF, Li XY, Chang M, Liu YF, Jin QZ, Wang XG (2015) A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources. Bioresour Technol 177:51鈥?7CrossRef
  
• 作者单位：Xue-Chao Hu (1)
  Lu-Jing Ren (1)
  Sheng-Lan Chen (1)
  Li Zhang (1)
  Xiao-Jun Ji (1)
  He Huang (1)
  
  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People鈥檚 Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

The effects of different osmotic pressure, changed by six salts (NaCl, Na₂SO₄, (NH₄)₂SO₄, KH₂PO₄ and MSG), on cell growth and DHA synthesis by Schizochytrium sp. were investigated. Six optimal mediums were obtained to study different osmotic pressure combinations at cell growth stage and DHA synthesis stage. Results showed that cultivated cell in higher osmotic pressure condition and fermented in lower osmotic pressure condition was benefit to enhance DHA synthesis. Combination 17-6 could get the maximum cell dry weight of 56.95 g/L and the highest DHA percentage in total fatty acids of 55.21 %, while combination 17-B could get the highest lipid yield of 33.47 g/L with 42.10 % DHA in total fatty acids. This was the first report about the enhancement of DHA production by osmotic regulation and this work provided two novel osmotic control processes for high lipid yield and high DHA percentage in total fatty acids. Keywords Schizochytrium sp. Osmotic pressure Docosahexaenoic acid Salts