Distribution and activity of anaerobic ammonium-oxidising bacteria in natural freshwater wetland soils
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- 作者：Li-dong Shen ; Hong-sheng Wu ; Zhi-qiu Gao…
- 关键词：Anammox activity ; Distribution ; Abundance ; Nitrogen loss ; Natural freshwater wetlands
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：100
- 期：7
- 页码：3291-3300
- 全文大小：486 KB
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Hong-sheng Wu (1)
Zhi-qiu Gao (2) (3)
Hai-xiang Cheng (4)
Ji Li (1)
Xu Liu (1)
Qian-qi Ren (1)
1. Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Jiangsu Key Laboratory of Agricultural Meteorology, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing, 210044, China
2. State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Science, Beijing, China
3. College of Geophysics and Remote Sensing, Nanjing University of Information Science and Technology, Nanjing, 210044, China
4. College of Chemistry and Materials Engineering, Quzhou University, Quzhou, 324000, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
Anaerobic ammonium oxidation (anammox) process plays a significant role in the marine nitrogen cycle. However, the quantitative importance of this process in nitrogen removal in wetland systems, particularly in natural freshwater wetlands, is still not determined. In the present study, we provided the evidence of the distribution and activity of anammox bacteria in a natural freshwater wetland, located in southeastern China, by using 15N stable isotope measurements, quantitative PCR assays and 16S rRNA gene clone library analysis. The potential anammox rates measured in this wetland system ranged between 2.5 and 25.5 nmol N2 g−1 soil day−1, and up to 20 % soil dinitrogen gas production could be attributed to the anammox process. Phylogenetic analysis of 16S rRNA genes showed that anammox bacteria related to Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus and two novel anammox clusters coexisted in the collected soil cores, with Candidatus Brocadia and Candidatus Kuenenia being the dominant anammox genera. Quantitative PCR of hydrazine synthase genes showed that the abundance of anammox bacteria varied from 2.3 × 105 to 2.2 × 106 copies g−1 soil in the examined soil cores. Correlation analyses suggested that the soil ammonium concentration had significant influence on the activity of anammox bacteria. On the basis of 15N tracing technology, it is estimated that a total loss of 31.1 g N m−2 per year could be linked the anammox process in the examined wetland.