Optimization of production conditions for antioxidant peptides from walnut protein meal using solid-state fermentation
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  • 作者:Wanxing Wu (1)
    Shenglan Zhao (2)
    Chaoyin Chen (1)
    Feng Ge (1)
    Diqiu Liu (1)
    Xiaoming He (2)
  • 关键词:walnut ; protein ; fermentation ; peptides ; antioxidant
  • 刊名:Food Science and Biotechnology
  • 出版年:2014
  • 出版时间:December 2014
  • 年:2014
  • 卷:23
  • 期:6
  • 页码:1941-1949
  • 全文大小:519 KB
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  • 作者单位:Wanxing Wu (1)
    Shenglan Zhao (2)
    Chaoyin Chen (1)
    Feng Ge (1)
    Diqiu Liu (1)
    Xiaoming He (2)

    1. Faculty of Life Science, Kunming University of Science and Technology, Kunming, 650500, China
    2. Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China
  • ISSN:2092-6456
文摘
Solid-state fermentation (SSF) of walnut protein meal (WPM) by Bacillus subtilis was optimized for the maximum degree of hydrolysis (DH) and reducing power (RP) using response surface methodology (RSM). Optimum fermentation conditions were fermentation time of 82.01 h, inoculum concentration of 10.40, and water content of 1.50 mL/g. Optimized values were 41.80% and 0.78 for DH and RP, respectively. Walnut peptides (WP) were ultrafiltration membrane fractionated. The WPs-II (molecular weight 2+ chelating activities, higher than for GSH (l-glutathione reduced) at the same concentration. Amino acid composition analysis of WPs-II showed that Asp, Glu, and Arg were the major amino acids playing important roles in the antioxidant activity. WPs is an efficient antioxidant suitable for use as a food additive and as a pharmaceutical agent.

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