Effect of LIM kinase 1 overexpression on behaviour of endometriosis-derived stromal cells

详细信息    查看全文

• 作者：Zhifang Zhang (1)
  Peng Chen (1)
  Cuishan Guo (1)
  Xiannan Meng (1)
  Danbo Wang (1)
  
  1. Department of Obstetrics and Gynecology ; Shengjing Hospital of China Medical University ; 36 Sanhao Street ; Shenyang ; 110004 ; People鈥檚 Republic of China
  
• 关键词：LIMK1 ; Endometriosis ; Eutopic endometrium ; Migration ; Invasion ; Proliferation ; Human
• 刊名：Cell and Tissue Research
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：359
• 期：3
• 页码：885-893
• 全文大小：5,705 KB
• 参考文献：1. Arber, S, Barbayannis, FA, Hanser, H, Schneider, C, Stanyon, CA, Bernard, O, Caroni, P (1998) Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase. Nature 393: pp. 805-809 CrossRef
  2. Chen, Q, Zhang, C, Chen, Y, Lou, J, Wang, D (2012) Identification of endometriosis-related genes by representational difference analysis of cDNA. Aust N Z J Obstet Gynaecol 52: pp. 140-145 CrossRef
  3. Chung, HW, Wen, Y, Chun, SH, Nezhat, C, Woo, BH, Lake Polan, M (2001) Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 mRNA expression in ectopic and eutopic endometrium in women with endometriosis: a rationale for endometriotic invasiveness. Fertil Steril 75: pp. 152-159 CrossRef
  4. C么t茅, MC, Lavoie, JR, Houle, F, Poirier, A, Rousseau, S, Huot, J (2010) Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1. J Biol Chem 285: pp. 8013-8021 CrossRef
  5. Davila, M, Frost, AR, Grizzle, WE, Chakrabarti, R (2003) LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: implications in prostate cancer. J Biol Chem 278: pp. 36868-36875 CrossRef
  6. Edwards, DC, Sanders, LC, Bokoch, GM, Gill, GN (1999) Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics. Nat Cell Biol 1: pp. 253-259 CrossRef
  7. Fazal, F, Bijli, KM, Minhajuddin, M, Rein, T, Finkelstein, JN, Rahman, A (2009) Essential role of cofilin-1 in regulating thrombin-induced RelA/p65 nuclear translocation and intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells. J Biol Chem 284: pp. 21047-21056 CrossRef
  8. Giudice, LC, Kao, LC (2004) Endometriosis. Lancet 364: pp. 1789-1799 CrossRef
  9. Giudice, LC, Tazuke, SI, Swiersz, L (1998) Status of current research on endometriosis. J Reprod 43: pp. 252-262
  10. Gonz谩lez-Ramos, R, Langendonckt, A, Defr猫re, S, Lousse, JC, Colette, S, Devoto, L, Donnez, J (2010) Involvement of the nuclear factor-魏B pathway in the pathogenesis of endometriosis. Fertil Steril 94: pp. 1985-1994 CrossRef
  11. Hirata, T, Osuga, Y, Takamura, M, Saito, A, Hasegawa, A, Koga, K, Yoshino, O, Hirota, Y, Harada, M, Taketani, Y (2011) Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis. Fertil Steril 96: pp. 113-117 CrossRef
  12. Kobayashi, M, Nishita, M, Mishima, T, Ohashi, K, Mizuno, K (2006) MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration. EMBO J 25: pp. 713-726 CrossRef
  13. Liu, H, Lang, JH (2011) Is abnormal eutopic endometrium the cause of endometriosis? The role of eutopic endometrium in pathogenesis of endometriosis. Med Sci Monit 17: pp. RA92-RA99
  14. Manetti, F (2012) LIM kinases are attractive targets with many macromolecular partners and only a few small molecule regulators. Med Res Rev 32: pp. 968-998 CrossRef
  15. Mishima, T, Naotsuka, M, Horita, Y, Sato, M, Ohashi, K, Mizuno, K (2010) LIM-kinase is critical for the mesenchymal-to-amoeboid cell morphological transition in 3D matrices. Biochem Biophys Res Commun 392: pp. 577-581 CrossRef
  16. Redwine, DB (2002) Was Sampson wrong ?. Fertil Steril 78: pp. 686-693 CrossRef
  17. Scott, RW, Olson, MF (2007) LIM kinases: function, regulation and association with human disease. J Mol Med (Berl) 85: pp. 555-568 CrossRef
  18. Starzinski-Powitz, A, Zeitvogel, A, Schreiner, A, Baumann, R (2001) In search of pathogenic mechanisms in endometriosis: the challenge for molecular cell biology. Curr Mol Med 1: pp. 655-664 CrossRef
  19. Sumi, T, Matsumoto, K, Nakamura, T (2002) Mitosis-dependent phosphorylation and activation of limkinase 1. Biochem Biophys Res Commun 290: pp. 1315-1320 CrossRef
  20. Swiersz, LM (2002) Role of endometriosis in cancer and tumor development. Ann N Y Acad Sci 955: pp. 281-292 CrossRef
  21. Tapia, T, Ottman, R, Chakrabarti, R (2011) LIM kinase1 modulates function of membrane type matrix metalloproteinase 1: implication in invasion of prostate cancer cells. Mol Cancer 10: pp. 6 CrossRef
  22. Tomiyoshi, G, Horita, Y, Nishita, M, Ohashi, K, Mizuno, K (2004) Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing. Genes Cells 9: pp. 591-600 CrossRef
  23. Vestergaard, AL, Thorup, K, Knudsen, UB, Munk, T, Rosbach, H, Poulsen, JB, Guldberg, P, Martensen, PM (2011) Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis. Mol Hum Reprod 17: pp. 758-761 CrossRef
  24. Wang, W, Goswami, S, Lapidus, K, Wells, AL, Wyckoff, JB, Sahai, E, Singer, RH, Segall, JE, Condeelis, JS (2004) Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors. Cancer Res 64: pp. 8585-8594 CrossRef
  25. Wang, W, Eddy, R, Condeelis, J (2007) The cofilin pathway in breast cancer invasion and metastasis. Nat Rev Cancer 7: pp. 429-440 CrossRef
  26. Yang, N, Higuchi, O, Ohashi, K, Nagata, K, Wada, A, Kangawa, K, Nishida, E, Mizuno, K (1998) Cofilin phosphorylation by LIM-kinase 1 and its role in Rac mediated actin reorganization. Nature 393: pp. 809-812 CrossRef
  27. Yan-Li, X, Wang, D-B, Liu, Q-F (2010) Silencing of cofilin-1 gene attenuates biological behaviours of stromal cells derived from eutopic endometria of women with endometriosis. Hum Reprod 25: pp. 2480-2488 CrossRef
  28. Yoshioka, K, Foletta, V, Bernard, O, Itoh, K (2003) A role for LIM kinase in cancer invasion. Proc Natl Acad Sci U S A 100: pp. 7247-7252 CrossRef
  29. Zhou, WD, Chen, QH, Chen, QX (2010) The action of p38 MAP kinase and its inhibitors on endometriosis. Yao Xue Xue Bao 45: pp. 548-554
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

LIM kinase 1 (LIMK1) has been implicated in tumour invasion and migration in several tumour types. However, its role in the progression of endometriosis has not yet been studied. Our aim is to analyse LIMK1 expression in endometriosis-derived stromal cells and to explore the effects of LIMK1 overexpression on their biological behaviour. The mRNA and protein expression levels of LIMK1 of eutopic endometrial stromal cells (ESCs) separated from 30 endometriosis patients and normal endometrial stromal cells (NSCs) separated from 30 patients without endometriosis were analysed by real-time polymerase chain reaction and Western blot. Lentiviral particles containing human LIMK1 short interfering RNA were used to silence the LIMK1 gene in ESCs and LIMK1-expressing lentiviral particles containing the total LIMK1 cDNA was transfected into NSCs to upregulate LIMK1 expression. Cell migration, invasion, proliferation and expression of markers of adhesion, invasion and angiogenesis of ESCs and NSCs were evaluated under basal conditions and after transfection in vitro. The mRNA and protein expression levels of LIMK1 in ESCs were higher than those in NSCs. Under basal conditions, ESCs exhibited greater cell migration, invasion and proliferation and higher levels of markers of adhesion, invasion and angiogenesis than NSCs. The behaviour of ESCs was decreased after LIMK1 gene silencing and that of NSCs was elevated after LIMK1 gene upregulation. Thus, LIMK1 is overexpressed in ESCs thereby facilitating malignant-like behaviour, including enhanced migration, invasion, proliferation and angiogenesis, all of which contribute to the occurrence and development of endometriosis.