Functional analyses of the maize CKS2 gene promoter in response to abiotic stresses and hormones

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• 作者：Fengting Wang (1)
  Jinliang Liu (1)
  Jingtao Li (1)
  Shihong Zhang (1)
  Hongyu Pan (1)
  
• 关键词：Promoter ; ZmCKS2 ; Transgenic Arabidopsis ; Abiotic stresses ; Hormone
• 刊名：Acta Physiologiae Plantarum
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：36
• 期：7
• 页码：1867-1878
• 全文大小：1,709 KB
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• 作者单位：Fengting Wang (1)
  Jinliang Liu (1)
  Jingtao Li (1)
  Shihong Zhang (1)
  Hongyu Pan (1)
  
  1. College of Plant Sciences, Jilin University, Changchun, 130062, China
  
• ISSN：1861-1664

文摘

In our previous research, we showed that the cyclin-dependent kinase regulatory subunit (CKS2) in maize (Zea mays L.) was induced by water deficit and cold stress. To elucidate its expression patterns under adversity, we isolated and characterized its promoter (PZmCKS2). A series of PZmCKS2-deletion derivatives, P0–P3, from the translation start code (?,455, ?99, ?67, and ??bp) was fused to the β-glucuronidase (GUS) reporter gene, and each deletion construct was analyzed by Agrobacterium-mediated steady transformation into Arabidopsis. Leaves were then subjected to dehydration, cold, abscisic acid (ABA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Sequence analysis showed that several stress-related cis-acting elements (MBS, CE3, TGA element, and ABRE) were located within the promoter. Deletion analysis of the promoter, PZmCKS2, suggested that the ?99?bp promoter region was required for the highest basal expression of GUS, and the ?67?bp sequence was the minimal promoter for ZmCKS2 activation by low temperature, ABA, and MeJA. The cis-acting element ABRE was necessary for promoter activation by exogenous ABA.