Molecular analysis of squalene epoxidase gene from Chlorophytum borivilianum (Sant. and Fernand.)

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• 作者：Shikha Kalra ; Sunil Kumar ; Kashmir Singh
• 关键词：Chlorophytum borivilianum ; Squalene epoxidase ; Saponins ; Gene expression
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：24
• 期：4
• 页码：417-424
• 全文大小：612 KB
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• 作者单位：Shikha Kalra (1)
  Sunil Kumar (1)
  Kashmir Singh (1)
  
  1. Department of Biotechnology, Panjab University, Chandigarh, 160014, India
  
• 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
• 出版者：Springer India
• ISSN：0974-1275

文摘

The present report describes the cloning, characterization and expression analysis of squalene epoxidase (SE) gene from C. borivilianum (Sant. and Fernand.). SE catalyzes the stereospecific epoxidation of squalene to 2, 3-oxidosqualene and is one of the rate limiting enzymes in the biosynthesis of saponins. A full length cDNA of CbSE contained 1,893 bp with an open reading frame of 1,599 bp, encoding a protein of 533 amino acids with conserved FAD, NAD(P) and squalene epoxidase domains. Phylogenetic analysis and 3D structure prediction using various programs showed CbSE structure to be similar to SE from other species. Open Reading Frame of SE was expressed in E. coli M15 cells, induced with 1 mM IPTG at 37 °C. Comparative expression analysis by semi-quantitative PCR demonstrated the high transcript levels of CbSE in leaf tissues as compared to root tissues. Keywords Chlorophytum borivilianum Squalene epoxidase Saponins Gene expression