Localized donor-acceptor pairs of fluorophores: Determination of the energy transfer rate by nonlinear fluorimetry

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• 作者：E. A. Shirshin (1) shirshin@lid.phys.msu.ru
  A. A. Banishev (1)
  V. V. Fadeev (1)
• 关键词：PACS numbers 33.15.Hp ; 42.62.Fi
• 刊名：JETP Letters
• 出版年：2009
• 出版时间：July 2009
• 年：2009
• 卷：89
• 期：10
• 页码：475-478
• 全文大小：151.2 KB

文摘

A laser fluorescence method has been developed for determining the energy transfer rate. It is based on the model of collective states of localized donor-acceptor pairs of fluorophores proposed in our previous work and involves nonlinear fluorimetry (other photophysical parameters of the donor and acceptor are simultaneously determined). The proposed approach has been tested on mRFP1 fluorescent (red) protein.