Localized donor-acceptor pairs of fluorophores: Determination of the energy transfer rate by nonlinear fluorimetry

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• 作者：E. A. Shirshin (1) shirshin@lid.phys.msu.ru
  A. A. Banishev (1)
  V. V. Fadeev (1)
• 关键词：PACS numbers 33.15.Hp ; 42.62.Fi
• 刊名：JETP Letters
• 出版年：2009
• 出版时间：July 2009
• 年：2009
• 卷：89
• 期：10
• 页码：475-478
• 全文大小：151.2 KB
• 参考文献：1. V. M. Agranovich and M. D. Galanin, Electronic Excitation Energy Transfer in Condensed Matter (Nauka, Moscow, 1978; North-Holland, Amsterdam, 1982).
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• 作者单位：1. Moscow State University Faculty of Physics Moscow 119991 Russia
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Elementary Particles and Nuclei
  Atoms, Molecules, Clusters and Plasmas
  Solid State Physics and Spectroscopy
  Russian Library of Science
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1090-6487

文摘

A laser fluorescence method has been developed for determining the energy transfer rate. It is based on the model of collective states of localized donor-acceptor pairs of fluorophores proposed in our previous work and involves nonlinear fluorimetry (other photophysical parameters of the donor and acceptor are simultaneously determined). The proposed approach has been tested on mRFP1 fluorescent (red) protein.