Generation of disk-like hydrogel beads for cell encapsulation and manipulation using a droplet-based microfluidic device

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• 作者：Kan Liu (1)
  Yuliang Deng (2) (3)
  Nangang Zhang (3)
  Sizhe Li (3)
  Huijiang Ding (3)
  Feng Guo (3)
  Wei Liu (3)
  Shishang Guo (3)
  Xing-Zhong Zhao (3)
  
• 关键词：Microfluidic device ; Disk ; like hydrogel beads ; Cell encapsulation ; Cell division observation
• 刊名：Microfluidics and Nanofluidics
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：13
• 期：5
• 页码：761-767
• 全文大小：490KB
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• 作者单位：Kan Liu (1)
  Yuliang Deng (2) (3)
  Nangang Zhang (3)
  Sizhe Li (3)
  Huijiang Ding (3)
  Feng Guo (3)
  Wei Liu (3)
  Shishang Guo (3)
  Xing-Zhong Zhao (3)
  
  1. School of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan, 430073, People鈥檚 Republic of China
  2. Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, People鈥檚 Republic of China
  3. Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, People鈥檚 Republic of China
  
• ISSN：1613-4990

文摘

We report a droplet-based microfluidic synthetic technique to generate disk-like hydrogel beads for cell encapsulation and manipulation. Utilizing this microfluidic synthetic technique, the size of the disk-like calcium alginate (CA) hydrogel beads and the number of cells encapsulated in the disk-like CA hydrogel beads could be well controlled by individually adjusting the flow rates of reagents. As a proof-of-concept, we demonstrated that single cell (yeast cell or mammalian cell) could be successfully encapsulated into disk-like CA hydrogel beads with high cell viability. Taking advantage of the flat top/bottom surfaces of disk-like CA hydrogel beads, cell division processes in culture media were clearly observed and recorded at a desired position without rolling and moving. This facile microfluidic chip provides a feasible method for size-controlled disk-like hydrogel beads generation and cell encapsulation. It could be a promising candidate for cell division observation and quantitative biological study in lab-on-a-chip applications.