Biocompatibility of polyhydroxybutyrate microspheres: in?vitro and in?vivo evaluation

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• 作者：Ekaterina Igorevna Shishatskaya ; Olga N. Voinova ; Anastasiya V. Goreva ; Olga A. Mogilnaya and Tatiana G. Volova
• 刊名：Journal of Materials Science Materials in Medicine
• 出版年：2008
• 出版时间：June, 2008
• 年：2008
• 卷：19
• 期：6
• 页码：2493-2502
• 全文大小：502.8 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biomaterials
  Characterization and Evaluation Materials
  Polymer Sciences
  Metallic Materials
  Ceramics,Glass,Composites,Natural Materials
  Surfaces and Interfaces and Thin Films
  
• 出版者：Springer Netherlands
• ISSN：1573-4838

文摘

Microspheres have been prepared from the resorbable linear polyester of β-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.