Polar protein transport between apical and basal cells during tobacco early embryogenesis

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• 作者：Shisheng Li (1) (2)
  Yuqing He (1)
  Jing Zhao (1)
  Liyao Zhang (1)
  Meng-xiang Sun (1)
  
• 关键词：Photoactivatable green fluorescent protein (PA ; GFP) ; Cell ; to ; cell communication ; Plasmodesmata ; Apical cell ; Basal cell ; Protein trafficking
• 刊名：Plant Cell Reports
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：32
• 期：2
• 页码：285-291
• 全文大小：426KB
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• 作者单位：Shisheng Li (1) (2)
  Yuqing He (1)
  Jing Zhao (1)
  Liyao Zhang (1)
  Meng-xiang Sun (1)
  
  1. State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, 430072, China
  2. Laboratory of Brassicaceae, Wuhan Institute of Vegetable Science, Wuhan, 430070, China
  
• ISSN：1432-203X

文摘

Key message We found that protein trafficking between apical and basal cell can be unidirectional, which reveals the different roles of the two cells in the cell-to-cell communication between them during early embryogenesis. Abstract In most angiosperm species, asymmetric zygote division results in an apical cell and a basal cell that have distinct cell fates. Much has been speculated about possible communication between these cell types in relation to their cell fate determination. Here, we report on the use of photoactivatable green fluorescent protein (PA-GFP) in tobacco to trace intercellular communication between apical and basal cells during early embryogenesis. We found that PA-GFP was transported between apical and basal cells of a two-celled proembryo, and that protein trafficking was unidirectional toward the apical cell, highlighting different cell communication roles. Further ultrastructural analysis showed numerous plasmodesmata in the walls connecting the apical and basal cells, which may provide channels for protein trafficking. Our data show a possible unique method of cell-to-cell communication between apical and basal cells during early embryogenesis.