Development and characterization of fifteen novel microsatellite loci for the Nassau grouper (Epinephelus striatus) and their utility for cross-amplification on a suite of closely related species

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• 作者：A. M. Bernard (1) (2)
  K. A. Feldheim (3)
  V. P. Richards (1) (2) (5)
  R. S. Nemeth (4)
  M. S. Shivji (1) (2)
  
• 关键词：Grouper genetics ; Grouper conservation ; Serranidae ; Epinephelus ; Mycteroperca
• 刊名：Conservation Genetics Resources
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：4
• 期：4
• 页码：983-986
• 全文大小：163KB
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• 作者单位：A. M. Bernard (1) (2)
  K. A. Feldheim (3)
  V. P. Richards (1) (2) (5)
  R. S. Nemeth (4)
  M. S. Shivji (1) (2)
  
  1. National Coral Reef Institute, Oceanographic Center, Nova Southeastern University, 8000 North Ocean Drive, Dania Beach, FL, 33004, USA
  2. Guy Harvey Research Institute, Oceanographic Center, Nova Southeastern University, 8000 North Ocean Drive, Dania Beach, FL, 33004, USA
  3. The Field Museum of Natural History, Pritzker Laboratory for Molecular Systematics and Evolution, 1400 South Lake Shore Drive, Chicago, IL, 60605, USA
  5. Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA
  4. Center for Marine and Environmental Studies, University of the Virgin Islands, St. Thomas, USVI, 00802, USA
  

文摘

The Nassau grouper (Epinephelus striatus), a severely overexploited and endangered coral reef fish in the Western Atlantic, is in urgent need of conservation and management measures. We report the development and characterization of 15 polymorphic, mostly tetranucleotide microsatellite loci based on 40 Nassau grouper samples collected from two Caribbean spawning aggregations. The number of alleles per locus and expected heterozygosities ranged from 4 to 19 and 0.40 to 0.92, respectively. We also demonstrate the cross-amplification utility of these microsatellites for genetic studies of five other grouper species of conservation interest.