Development and characterization of thirteen microsatellite markers for the longbill spearfish (Tetrapturus pfluegeri)

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• 作者：A. M. Bernard (1)
  A. W. S. Hilsdorf (2)
  A. F. Amorim (3)
  R. R. Domingues (2)
  M. S. Shivji (1)
  
• 关键词：Billfish genetics ; Billfish conservation and management ; Istiophoridae ; STR
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：6
• 期：1
• 页码：169-171
• 全文大小：156 KB
• 作者单位：A. M. Bernard (1)
  A. W. S. Hilsdorf (2)
  A. F. Amorim (3)
  R. R. Domingues (2)
  M. S. Shivji (1)
  
  1. Guy Harvey Research Institute, Oceanographic Center, Nova Southeastern University, 8000 North Ocean Drive, Dania Beach, FL, 33004, USA
  2. Unit of Biotechnology, University of Mogi das Cruzes, PO Box 411, Mogi das Cruzes, SP, 08701-970, Brazil
  3. Fisheries Institute, Agência Paulista de Tecnologia dos Agronegócios, Av. Bartolomeu de Gusm?o, 192, Santos, SP, 11030-906, Brazil
  
• ISSN：1877-7260

文摘

The longbill spearfish (Tetrapturus pfluegeri; Istiophoridae) is an epipelagic billfish species broadly distributed in the Atlantic Ocean and exploited by multinational fisheries. No data exist on the population structure or genetic diversity of this apex predator, despite a strong need for this information to inform international conservation and management efforts. Thirteen microsatellite loci, comprising mostly tri- and tetra-nucleotide repeats, were isolated and characterized for this species by genotyping individuals (n?=?29-2) obtained from western North and South Atlantic fisheries. The average number of alleles ranged from 3 to 16 and the expected heterozygosity ranged from 0.35 to 0.86. This suite of markers provides the first population genetic nuclear resources for the longbill spearfish, allowing initiation of studies for assessing the population structure and demography of this poorly understood species.