Characterization of one novel cry8 gene from Bacillus thuringiensis strain Q52-7

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• 作者：Haitao Li (1) (2)
  Rongmei Liu (1)
  Changlong Shu (2)
  Qingli Zhang (1)
  Shiyuan Zhao (1)
  Gaoxiang Shao (1)
  Xiaofeng Zhang (1)
  Jiguo Gao (1)
  
• 关键词：Bacillus thuringiensis ; Cry8Na1 gene ; Holotrichia parallela
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：30
• 期：12
• 页码：3075-3080
• 全文大小：524 KB
• 参考文献：1. Abad AR, Duck NB, Feng X, Flannagan RD, Kahn TW, Sims LE (2002) Genes encoding novel proteins with pesticidal activity against coleopterans. WO 02/34774 A2. 02 May 2002. E.I. DuPont de Nemours and company, USA
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• 作者单位：Haitao Li (1) (2)
  Rongmei Liu (1)
  Changlong Shu (2)
  Qingli Zhang (1)
  Shiyuan Zhao (1)
  Gaoxiang Shao (1)
  Xiaofeng Zhang (1)
  Jiguo Gao (1)
  
  1. Northeast Agricultural University, Harbin, 150030, People’s Republic of China
  2. State Key Laboratory of Biology for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100094, People’s Republic of China
  
• ISSN：1573-0972

文摘

Bacillus thuringiensis (Bt) is the most widely used insecticidal microbe due to its specific toxicity and safe use with respect to animals and the environment. In this study, we isolated Bt strain Q52-7 from a soil sample collected in the Qian Shan District, Liao Ning Province, China. We observed that the Q52-7 strain produced spherical crystals. The Bt Q52-7 strain had high toxicity against Asian Cockchafer (Holotrichia parallela), exhibiting an LC50 of 3.80?×?109 cfu/g, but is not toxic for Anomala corpulenta Motschulsky and Holotrichia oblita. Using general cry8 primers, we amplified a 1.3?kb fragment with the polymerase chain reaction. Specific primers were designed for the amplified fragment to clone the full-length coding region. A novel gene, cry8Na1, had 69?% sequence similarity with cry8Ca1. cry8Na1 gene was successfully expressed in the HD-73-/sup> acrystalliferous mutant of Bt subsp. Kurstaki HD-73. Bioassays demonstrated that the Cry8Na1 protein is highly toxic for the H. parallela, with a 50?% lethal concentration of 8.18?×?1010 colony forming units per gram.