Ameliorative effects of Eriobotrya japonica seed extract on cellular aging in cultured rat fibroblasts
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- 作者：Kazuyo Muramoto (1)
Rong-Dan Quan (2)
Toshiharu Namba (2)
Shojiro Kyotani (3)
Mitsuhiko Miyamura (4)
Yutaka Nishioka (4)
Keiichi Tonosaki (1)
Yoshinori L. Doi (5)
Hideto Kaba (2) - 关键词：Loquat seed extract ; Cellular senescence ; Calcium imaging ; Intracellular calcium dynamics ; Fibroblast ; Bradykinin
- 刊名：Journal of Natural Medicines
- 出版年：2011
- 出版时间：April 2011
- 年：2011
- 卷：65
- 期：2
- 页码：254-261
- 全文大小：425KB
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Rong-Dan Quan (2)
Toshiharu Namba (2)
Shojiro Kyotani (3)
Mitsuhiko Miyamura (4)
Yutaka Nishioka (4)
Keiichi Tonosaki (1)
Yoshinori L. Doi (5)
Hideto Kaba (2)
1. Division of Physiology, Department of Human Development and Fostering, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 350-0283, Japan
2. Department of Physiology, Kochi Medical School, Nankoku, Kochi, 783-8505, Japan
3. Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, 770-8514, Japan
4. Department of Pharmacy, Kochi Medical School Hospital, Nankoku, Kochi, 783-8505, Japan
5. Department of Medicine and Geriatrics, Kochi Medical School, Nankoku, Kochi, 783-8505, Japan - ISSN：1861-0293
文摘
To investigate the effects of Eriobotrya japonica seed extract (ESE) on cellular aging, intracellular calcium homeostasis in young and senescent cells was analyzed using a rat fibroblast culture as an in vitro model system and a calcium imaging technique. The application of bradykinin (BK) transiently elicited intracellular calcium ion (Ca2+) increased in most of the young fibroblasts, whereas these responses were scarcely observed or were significantly attenuated in senescent cells. However, the long-term treatment of senescent cells with ESE (for 7?days) dose-dependently increased the amplitude of BK-induced responses and the percentage of BK-responding cells. In particular, most senescent cells could respond to BK with long-term treatment with ESE (1.0% or 2.0%), an effect that reinstated the percentage of BK-responding cells to the same level as that in young cells. The effects of ESE on amplitude or percentage of responding cells were not observed in young cells. Moreover, the time to half decay, which was significantly longer in senescent cells than that in young cells, was shortened in senescent cells with long-term treatment with ESE. These results suggest that treatment with an adequate concentration of ESE renders BK-induced Ca2+ dynamics in senescent cells similar to those in young cells. Therefore, ESE can retard and/or protect against cellular aging and may be useful for elucidating the antiaging processes.