Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoene synthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis

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• 作者：Shota Kato ; Shinichi Takaichi ; Takahiro Ishikawa ; Masashi Asahina…
• 关键词：Euglena gracilis ; Light stress ; Carotenoid biosynthesis ; Geranylgeranyl pyrophosphate synthase ; CrtE ; Phytoene synthase ; CrtB
• 刊名：BMC Plant Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：2,830 KB
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• 作者单位：Shota Kato (1) (4)
  Shinichi Takaichi (2)
  Takahiro Ishikawa (3)
  Masashi Asahina (1)
  Senji Takahashi (1)
  Tomoko Shinomura (1) (4)
  
  1. Department of Biosciences, School of Science and Engineering, Teikyo University, 1-1 Toyosatodai, Utsunomiya, Tochigi, 320-8551, Japan
  4. Plant Molecular and Cellular Biology Laboratory, Department of Biosciences, School of Science and Engineering, Teikyo University, 1-1 Toyosatodai, Utsunomiya, Tochigi, 320-8551, Japan
  2. Department of Biology, Nippon Medical School, 1-7-1 Kyonan-cho, Musashino, Tokyo, 180-0023, Japan
  3. Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan
  
• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background Euglena gracilis, a unicellular phytoflagellate within Euglenida, has attracted much attention as a potential feedstock for renewable energy production. In outdoor open-pond cultivation for biofuel production, excess direct sunlight can inhibit photosynthesis in this alga and decrease its productivity. Carotenoids play important roles in light harvesting during photosynthesis and offer photoprotection for certain non-photosynthetic and photosynthetic organisms including cyanobacteria, algae, and higher plants. Although, Euglenida contains β-carotene and xanthophylls (such as zeaxanthin, diatoxanthin, diadinoxanthin and 9′-cis neoxanthin), the pathway of carotenoid biosynthesis has not been elucidated.