Indian hedgehog contributes to human cartilage endplate degeneration

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• 作者：Shaowei Wang ; Kun Yang ; Shuai Chen ; Jiying Wang ; Guoqing Du…
• 关键词：Indian hedgehog ; Cartilaginous endplate ; Intervertebral disc degeneration ; Low back pain
• 刊名：European Spine Journal
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：24
• 期：8
• 页码：1720-1728
• 全文大小：5,111 KB
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• 作者单位：Shaowei Wang (1)
  Kun Yang (2)
  Shuai Chen (1)
  Jiying Wang (1)
  Guoqing Du (2)
  Shunwu Fan (1)
  Lei Wei (2)
  
  1. Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, NO.3 East Qingchun Road, Hangzhou, 310016, China
  2. Department of Orthopaedics, Warren Alpert Medical School of Brown University and Rhode Island Hospital, Providence, USA
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Surgical Orthopedics
  Neurosurgery
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0932

文摘

Purpose To determine the role of Indian hedgehog (Ihh) signaling in human cartilage endplate (CEP) degeneration. Methods CEP-degenerated tissues from patients with Modic I or II changes (n?=?9 and 45, respectively) and normal tissues from vertebral burst fracture patients (n?=?17) were collected. Specimens were either cut into slices for organ culture ex vivo or digested to isolate chondrocytes for cell culture in vitro. Ihh expression and the effect of Ihh on cartilage degeneration were determined by investigating degeneration markers in this study. Results Ihh expression and cartilage degeneration markers significantly increased in the Modic I and II groups. The expression of cartilage degeneration markers was positively correlated with degeneration severity. Gain-of-function for Ihh promoted expression of cartilage degeneration markers in vitro, while loss-of-function for Ihh inhibited their expression both in vitro and ex vivo. Conclusions These findings demonstrated that Ihh promotes CEP degeneration. Blocking Ihh pathway has potential clinical usage for attenuating CEP degeneration.