Mycobacteriophage putative GTPase-activating protein can potentiate antibiotics

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• 作者：Shuangquan Yan ; Mengmeng Xu ; Xiangke Duan…
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：100
• 期：18
• 页码：8169-8177
• 全文大小：1,852 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614
• 卷排序：100

文摘

The soaring incidences of infection by antimicrobial resistant (AR) pathogens and shortage of effective antibiotics with new mechanisms of action have renewed interest in phage therapy. This scenario is exemplified by resistant tuberculosis (TB), caused by resistant Mycobacterium tuberculosis. Mycobacteriophage SWU1 A321_gp67 encodes a putative GTPase-activating protein. Mycobacterium smegmatis with gp67 overexpression showed changed colony formation and biofilm morphology and supports the efficacy of streptomycin and capreomycin against Mycobacterium. gp67 down-regulated the transcription of genes involved in cell wall and biofilm development. To our knowledge, this is the first report to show that phage protein in addition to lysin or recombination components can synergize with existing antibiotics. Phage components might represent a promising new clue for better antibiotic potentiators.KeywordsMycobacteriophageAntimicrobial resistanceBiofilmGTPase-activating proteinAntibiotic potentiator